Stability of vancomycin-resistant enterococcal genotypes isolated from long-term-colonized patients
Stability of vancomycin-resistant enterococcal genotypes isolated from long-term-colonized patients
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DOI：
10.1086/514196
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发表时间：
1998-02-01
影响因子：
6.4
通讯作者：
Weinstein, RA
中科院分区：
文献类型：
--
作者：
Bonten, MJM;Hayden, MK;Weinstein, RA
为探讨万古霉素耐药肠球菌（VRE）的遗传多样性和脉冲场凝胶电泳（PFGE）菌株定义是否适用于地方性流行病，对455株VRE菌株进行了基因型变异和稳定性研究。在455株VRE菌株中鉴定出22种PFGE类型，10种vanA屎肠球菌菌株类型的一对一比较均产生>10条带差异，vanA和vanB E之间的变化。来自个体长期定殖（4-160天）患者的屎肠菌分离物产生85%的比较。未按万古霉素抗性类型分组的所有菌株的比较产生两个带差异峰：一个具有10个带差异。这些数据表明，VRE分离株在遗传上密切相关或非常不同;表明在个体患者中，VRE分离株显示很少的遗传变异;并提供经验证据，PFGE可用于研究VRE流行病学。
Genotypic variation and stability of isolates of vancomycin-resistant enterococci (VRE) were studied to determine genetic diversity and whether strain definition based on pulsed-field gel electrophoresis (PFGE) is applicable to an endemic setting, Twenty-two PFGE types were identified among 455 VRE isolates, One-on-one comparisons of 10 vanA Enterococcus faecium strain types all yielded >10 band differences, Variations among vanA and vanB E. faecium isolates from individual longterm-colonized (4-160 days) patients yielded 85% of comparisons, Comparison of all strains without grouping by vancomycin resistance types yielded two peaks of band differences: one with 10 band differences. These data show that VRE isolates were genetically closely related or very different; demonstrate that within individual patients, VRE isolates show Little genetic variation; and provide empirical evidence that PFGE can be used to study the epidemiology of VRE endemicity.