Enzymatic processes for biodegradation of poly(hydroxyalkanoate)s crystals
Enzymatic processes for biodegradation of poly(hydroxyalkanoate)s crystals
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DOI：
10.1139/v08-004
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发表时间：
2008-06
影响因子：
1.1
通讯作者：
K. Numata;H. Abe;Y. Doi
中科院分区：
文献类型：
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作者：
K. Numata;H. Abe;Y. Doi
聚羟基烷酸酯（PHAs）作为一种可利用可再生碳资源制备的环境相容性高分子材料，已引起人们的广泛关注。PHA材料的生物降解通过微生物分泌的细胞外PHA解聚酶的功能发生。因此，阐明PHA材料的酶促降解机制对于设计具有所需性质和可控生物降解性的PHA材料是重要的。固体PHA聚合物是水不溶性底物，但PHA解聚酶可溶于水。因此，PHA材料的酶促降解是材料表面上的非均相反应。降解过程涉及两个不同的过程，即酶在PHA材料表面的吸附和随后的聚合物链的水解。原子力显微镜（AFM）是一种强有力的工具，已被用于定量分析PHA晶体的降解。原子力显微镜能够表征晶体表面的纳米结构。
Poly(hydroxyalkanoate)s (PHAs) have attracted much attention as environmentally compatible polymeric materials that can be produced from renewable carbon resources. Biodegradation of PHA materials occurs by the function of extracellular PHA depolymerase secreted from microorganisms. Thus, elucidation of the enzymatic degradation mechanism for PHA materials is important to design PHA materials with desirable properties and controlled biodegradability. The solid PHA polymer is a water-insoluble substrate but PHA depolymerases are soluble in water. Therefore, the enzymatic degradation of PHA materials is a heterogeneous reaction on the material’s surface. Two distinct processes are involved during the degradation, namely, adsorption of the enzyme on the surface of PHA material and the subsequent hydrolysis of polymer chains. Atomic force microscopy (AFM) is a powerful tool that has been used for the quantitative analysis of PHA crystal degradation. AFM enables the characterization of the crystal surface nano...