Imprinting analysis of 10 genes and/or transcripts in a 1.5-Mb MEST-flanking region at human chromosome 7q32.
Imprinting analysis of 10 genes and/or transcripts in a 1.5-Mb MEST-flanking region at human chromosome 7q32.
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DOI：
10.1016/j.ygeno.2003.08.016
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发表时间：
2004-03
期刊：
影响因子：
4.4
通讯作者：
Takahiro Yamada;K. Mitsuya;Tomohiko Kayashima;K. Yamasaki;T. Ohta;K. Yoshiura;N. Matsumoto;Hideto Yamada;H. Minakami;M. Oshimura;N. Niikawa;T. Kishino
中科院分区：
文献类型：
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作者：
Takahiro Yamada;K. Mitsuya;Tomohiko Kayashima;K. Yamasaki;T. Ohta;K. Yoshiura;N. Matsumoto;Hideto Yamada;H. Minakami;M. Oshimura;N. Niikawa;T. Kishino
MEST是人类印迹基因之一。借助整合图谱和注册表中的完整序列，我们在7q32的1.5 mb mest侧翼区域共定位了16个基因/转录本。该区域被认为形成了一个印迹基因簇，因为据报道MEST及其三个侧翼基因/转录本（mesti1， CPA4和COPG2IT1）被印迹，尽管两个（TSGA14和COPG2）被证明没有印迹。在这项研究中，我们检测了另外10个基因/转录本在人类胎儿组织中的印迹状态。结果显示，8个基因/转录本（NRF1、UBE2H、HSPC216、KIAA0265、FLJ14803、CPA2、CPA1和DKFZp667F0312）双等位表达。两种基因（TSGA13和CPA5）的印迹状态尚不确定，因为它们的表达较弱和/或具有组织特异性，结果不稳定。这些发现提供了证据，表明位于该区域的16个基因/转录本中只有4个显示单等位基因表达，而其他基因/转录本不参与印迹。因此，mst侧7q32区域不太可能形成一个大的印迹结构域。
MEST is one of the imprinted genes in human. With the assistance of our integration map and the complete sequence in the registry, we mapped a total of 16 genes/transcripts at the 1.5-Mb MEST-flanking region at 7q32. This region has been suggested to form an imprinted gene cluster, because MEST and its three flanking genes/transcripts (MESTIT1, CPA4, and COPG2IT1) were reported to be imprinted, although two (TSGA14 and COPG2) were shown to escape imprinting. In this study, 10 other genes/transcripts were examined for their imprinting status in human fetal tissues. The results indicated that 8 genes/transcripts (NRF1, UBE2H, HSPC216, KIAA0265, FLJ14803, CPA2, CPA1, and DKFZp667F0312) were expressed biallelically. The imprinting status of two (TSGA13 and CPA5) was not conclusive, because of their weak and/or tissue-specific expression and inconstant results. These findings provided evidence that only 4 of the 16 genes/transcripts located to the region show monoallelic expression, while others are not involved in imprinting. Therefore, it is less likely that the MEST-flanking 7q32 region forms a large imprinted domain.