Experimental mouse models of ragweed- and papain-induced allergic conjunctivitis.
Experimental mouse models of ragweed- and papain-induced allergic conjunctivitis.
复制标题
豚草和木瓜蛋白酶诱导的过敏性结膜炎的实验小鼠模型。
DOI：
10.1007/978-1-0716-1001-5_10
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Asada Y
中科院分区：
文献类型：
--
作者：
Matsuda A;Hirakata T;Asada Y
过敏性结膜炎的小鼠模型模拟了人类过敏性结膜炎的各个方面。他们是有用的过敏性结膜炎的急性模型，以研究这种情况的免疫学方面。在本章中，我们将描述豚草花粉诱导的实验性过敏性结膜炎（主要由适应性免疫驱动）和木瓜浸泡接触镜诱导的实验性过敏性结膜炎（主要由先天免疫驱动）。吉姆萨染色用于定量组织学切片中浸润的嗜酸性粒细胞的数量，这有助于评估过敏性炎症的严重程度。利用免疫组织化学染色和定量PCR技术，明确了促炎分子在结膜组织中的时空表达。结膜组织流式细胞术用于检测眼表组织中先天性淋巴样细胞2型（ILC2）。
Mouse models of allergic conjunctivitis mimic various aspects of human allergic conjunctivitis. They are useful as acute models of allergic conjunctivitis to study immunological aspects of this condition. In this chapter, we will describe ragweed-pollen-induced experimental allergic conjunctivitis (mostly driven by adaptive immunity), and papain-soaked contact lens-induced experimental allergic conjunctivitis (mostly driven by innate immunity). Giemsa staining of histological sections is used for quantification of the number of infiltrating eosinophils, which is useful to evaluate the severity of the allergic inflammation. Immunohistochemical staining and quantitative PCR are used to clarify spatiotemporal expression of proinflammatory molecules in the conjunctival tissue. Flow cytometric analysis of conjunctival tissue is used for the detection of innate lymphoid cell type 2 (ILC2) in the ocular surface tissues.