THE DEMONSTRATION OF INSULIN-LIKE MATERIAL IN THE HONEY BEE, APIS-MELLIFERA
THE DEMONSTRATION OF INSULIN-LIKE MATERIAL IN THE HONEY BEE, APIS-MELLIFERA
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DOI：
10.1016/0305-0491(85)90400-6
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发表时间：
1985-01-01
影响因子：
2.2
通讯作者：
BAXTER, D
中科院分区：
文献类型：
--
作者：
OCONNOR, KJ;BAXTER, D
部分纯化的蜜蜂提取物（A. mellifera）和它们分离的头在猪胰岛素放射免疫测定中交叉反应，而去头体没有活性。活性提取物取代猪胰岛素从大鼠肝脏胰岛素受体，并显示与大鼠脂肪细胞的胰岛素样活性，可以取消与牛胰岛素抗血清。证实了皇家浆中含有胰岛素。蜜蜂头部中存在与哺乳动物非常相似的胰岛素，表明该分子具有高度进化保守性。
Partially purified extracts from bees (A. mellifera) and their separated heads cross-reacted in a porcine insulin radioimmunoassay whereas the decapitated bodies were devoid of activity. The active extracts displaced porcine insulin from rat liver insulin receptors and showed insulin-like activity with rat adipocytes which could be abolished with bovine insulin antiserum. The presence of insulin in bee royal jelly was demonstrated. An insulin of close similarity to mammalian is present in the head of the bee, indicating high evolutionary conservation of the molecule.