Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins.
Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins.
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DOI：
10.1038/nmeth.1808
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发表时间：
2011-12-18
期刊：
影响因子：
48
通讯作者：
Deisseroth, Karl
中科院分区：
文献类型：
--
作者：
Mattis, Joanna;Tye, Kay M.;Ferenczi, Emily A.;Ramakrishnan, Charu;O'Shea, Daniel J.;Prakash, Rohit;Gunaydin, Lisa A.;Hyun, Minsuk;Fenno, Lief E.;Gradinaru, Viviana;Yizhar, Ofer;Deisseroth, Karl
不同的光遗传工具可以对神经活动进行多种控制。许多去极化和超极化工具现在已经在多个实验室开发，并在不同的准备工作中进行测试，这既提供了机会，也使人们难以进行直接比较。这一挑战因载体、启动子、表达时间、光照、细胞类型和许多其他变量对性能的依赖而变得更加复杂。因此，对于最终用户来说，为他们的实验需要选择最佳试剂变得越来越复杂。对于一个迅速发展的领域，关键的优点系数应正规化，以便为进一步发展建立一个框架，并使最终用户能够容易地了解这些标准化参数如何转化为业绩。在这里，我们在匹配的实验条件下系统地比较微生物视蛋白，以提取基本原理并确定关键参数，以进行、设计和解释涉及光遗传技术的实验。
Diverse optogenetic tools have allowed versatile control over neural activity. Many depolarizing and hyperpolarizing tools have now been developed in multiple laboratories and tested across different preparations, presenting opportunities but also making it difficult to draw direct comparisons. This challenge has been compounded by the dependence of performance on parameters such as vector, promoter, expression time, illumination, cell type and many other variables. As a result, it has become increasingly complicated for end users to select the optimal reagents for their experimental needs. For a rapidly growing field, critical figures of merit should be formalized both to establish a framework for further development and so that end users can readily understand how these standardized parameters translate into performance. Here we systematically compared microbial opsins under matched experimental conditions to extract essential principles and identify key parameters for the conduct, design and interpretation of experiments involving optogenetic techniques.
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