Proposal of Pumpless, Valveless, and Flowless Miniaturized Reactor Using Magnetic Beads for the Portable Analysis Device
Proposal of Pumpless, Valveless, and Flowless Miniaturized Reactor Using Magnetic Beads for the Portable Analysis Device
复制标题
用于便携式分析装置的使用磁珠的无泵、无阀、无流小型化反应器的提案
DOI：
--
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
H. Honda
中科院分区：
文献类型：
--
作者：
Hiroshi Ito;K. Takayanagi;M. Okochi;M. Shikida;Kazuo Sato;H. Honda
研制了一种基于磁珠-液滴处理机构的小型化学分析系统。该分析系统没有复杂的机械部件，为便携式分析设备的发展提供了可能。在本报告中，我们构建了一个由8口井通过通道连接而成的分析系统。采用直径为22.0 μm的磁珠作为流动相。我们演示了使用该系统检测生物素标记的DNA低聚物。结果表明，该检测系统具有特异性和信号对目标DNA浓度的依赖性。
A miniaturized chemical-analysis system based on magnetic beads-droplet-handling mechanism without pumps and valves was developed for analysis. The analysis system has possibility for the development of a portable analysis device since there is no complicated mechanical component. In this report, we constructed an analysis system with 8 wells connected by channels. Magnetic beads with a diameter of 22.0 μm were used as a mobile phase. We demonstrated the detection of biotin-labeled DNA oligomers using this system. The result showed that this detection system has specificity and dependence of the signal on the target DNA concentration.