Lipid oxidation is reduced in obese human skeletal muscle
Lipid oxidation is reduced in obese human skeletal muscle
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DOI：
10.1152/ajpendo.2000.279.5.e1039
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发表时间：
2000-11-01
影响因子：
5.1
通讯作者：
Houmard, JA
中科院分区：
文献类型：
--
作者：
Kim, JY;Hickner, RC;Houmard, JA
本研究的目的是辨别有助于抑制肥胖个体骨骼肌脂质氧化的细胞机制。研究人员从肥胖女性（体重指数为38.3 +/- 3.1 kg/m(2)）和瘦弱女性（体重指数为23.8 +/- 0.9 kg/m(2)）中获得肌肉，并通过测量（14）c标记脂肪酸产生的（14）CO(2)来研究脂肪酸氧化。棕榈酸酯氧化至少部分依赖于肉碱棕榈酰基转移酶-1 （CPT-1）活性，肥胖导致50%的棕榈酸酯氧化被抑制（P < 0.05） (6.8 +/- 2.2 vs. 13.7 +/- 1.4 nmol CO(2).g（-1).h(-1)）。与cpt -1无关的棕榈酰肉碱氧化事件也降低了45% （P < 0.01）。脂肪与棕榈酸酯（r = -0.76）和棕榈酰肉碱（r = 0.82）氧化呈显著负相关（P < 0.05）。肌肉CPT-1和柠檬酸合成酶活性（线粒体含量指标）也随肥胖显著（P < 0.05）降低（约35%）。CPT-1 （r = -0.48）和柠檬酸合成酶（r = -0.65）活性与肥胖显著相关（P < 0.05）。这些数据表明，CPT-1和CPT-1事件后的病变，如线粒体含量，有助于减少对脂肪氧化的依赖，这在肥胖的人类骨骼肌中很明显。
The purpose of this study was to discern cellular mechanisms that contribute to the suppression of lipid oxidation in the skeletal muscle of obese individuals. Muscle was obtained from obese [body mass index (BMI), 38.3 +/- 3.1 kg/m(2)] and lean (BMI, 23.8 +/- 0.9 kg/m(2)) women, and fatty acid oxidation was studied by measuring (14)CO(2) production from (14)C-labeled fatty acids. Palmitate oxidation, which is at least partially dependent on carnitine palmitoyltransferase-1 (CPT-1) activity, was depressed (P < 0.05) by 50% with obesity (6.8 +/- 2.2 vs. 13.7 +/- 1.4 nmole CO(2).g(-1).h(-1)). The CPT-1-independent event of palmitoyl carnitine oxidation was also depressed (P < 0.01) by 45%. There were significant negative relationships (P < 0.05) for adiposity with palmitate (r = -0.76) and palmitoyl carnitine (r = 0.82) oxidation. Muscle CPT-1 and citrate synthase activity, an index of mitochondrial content, were also significantly (P < 0.05) reduced (approximate to 35%) with obesity. CPT-1 (r = -0.48) and citrate synthase (r = -0.65) activities were significantly (P < 0.05) related to adiposity. These data suggest that lesions at CPT-1 and post-CPT-1 events, such as mitochondrial content, contribute to the reduced reliance on fat oxidation evident in human skeletal muscle with obesity.