Expression of proliferating cell nuclear antigen in pulp cells of extracted immature teeth preserved in two different storage media.
Expression of proliferating cell nuclear antigen in pulp cells of extracted immature teeth preserved in two different storage media.
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保存在两种不同存储介质中的提取的未成熟牙齿的牙髓细胞中增殖细胞核抗原的表达。
DOI：
10.1111/j.1600-9657.2005.00400.x
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
H. Kirschner
中科院分区：
文献类型：
--
作者：
U. Tekin;A. Filippi;Y. Pohl;H. Kirschner
研制了一种特殊成分的脱位牙保存介质。在实验和临床研究中，PDL细胞在培养液中可以保持存活长达53h。在本研究中，该培养液在牙髓细胞上进行了测试。手术拔除40颗未成熟未萌出根尖的第三磨牙，将其置于特殊细胞培养液(SCCM)或Hank平衡盐溶液(HBSS)中室温保存6、12、18、24 h，每组5颗。共检查了7个连续的每颗牙的牙髓横断面，总共产生了280个标本。活细胞用增殖细胞核抗原(PCNA)标记。牙髓分为3个区域：顶区(0~0.5 mm)、中区(0.5~1.5 mm)和冠区(1.5 mm)。分别计算整个切面(区域1、2、3)和每个区域的标记指数(LI)。统计评价采用单因素方差和Mann-Whitney检验。所有牙齿的牙髓细胞均表达增殖细胞核抗原。鳞状细胞癌140例中约110例阳性细胞，HBSS组140例中101例阳性细胞。最高LI出现在根尖区，并随着距离介质距离的增加而降低。在超过1.5 mm的距离处，未观察到标记细胞。随着保存时间的延长，两种介质的LI均显著增加(P<；0.05)。在根尖和中段，SCCM保存的牙髓细胞的LI几乎是HBSS保存的牙髓细胞的两倍(时间间隔6、18和24小时：P<；0.05)。24 h后，SCCM的LI为8.43%，HBSS的LI为4.50%，中部SCCM的LI为2.02%，HBSS的LI为0.81%。在本研究的参数范围内，SCCM比HBSS更能维持牙髓细胞的活性。在牙齿撕脱的情况下，使用特殊的细胞培养液可能是有益的。
A specially composed medium for storing avulsed teeth has been developed. In experimental and clinical studies it could be shown that PDL cells could be kept viable during storage in the medium for up to 53 h. In the present study the medium was tested on pulp cells. A total of 40 immature unerupted third molars with open apices were removed surgically and the teeth were stored in a special cell culture medium (SCCM) or in Hank's balanced salt solution (HBSS) at room temperature for 6, 12, 18 or 24 h. Five teeth were assigned to each group. A total of seven consecutive pulp cross-sections per tooth were examined, resulting in a total of 280 specimens. Viable cells were marked using proliferating cell nuclear antigen (PCNA). The pulp was divided in three regions: apical region (0-0.5 mm), middle region (>0.5-1.5 mm) and coronal region (>1.5 mm). The labelling index (LI) was calculated for the whole cut (regions 1, 2 and 3) and for each region separately. The statistical evaluation was made using the One-way anova and Mann-Whitney Test. Pulp cells of all teeth expressed PCNA. About 110 of 140 specimens in the SCCM and 101 of 140 specimens in the HBSS group showed PCNA-positive cells. The highest LI was observed within the apical region and decreased with increased distance from the medium. No marked cells were observed at a distance of more than 1.5 mm. The LI for both media showed a significant increase with storage intervals (P < 0.05). The pulp cells of teeth stored in SCCM showed a LI nearly twice as high compared to pulp cells of teeth stored in HBSS for the apical and middle region (time interval 6, 18 and 24 h: P < 0.05). The LI for the apical region was found to be 8.43% for the SCCM and 4.50% for the HBSS after 24 h. For the middle region the LI was found to be 2.02% for the SCCM and 0.81% for the HBSS after 24 h. Within the parameters of this study, it appears that the SCCM is able to maintain pulp cell viability better than HBSS. The use of special cell culture media in case of tooth avulsion may be beneficial.