Evaluation of the effect of cadmium on rat organs and investigation of diethyl carbamate as an oral drug in treatment of cadmium toxicity
Evaluation of the effect of cadmium on rat organs and investigation of diethyl carbamate as an oral drug in treatment of cadmium toxicity
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DOI：
10.1007/s00044-008-9118-z
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发表时间：
2009-03
影响因子：
2.6
通讯作者：
S. Jamilaldine Fatemi;S. Tubafard;B. Nadi
中科院分区：
文献类型：
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作者：
S. Jamilaldine Fatemi;S. Tubafard;B. Nadi
在给予镉中毒的 Wistar 大鼠食物和饮料后，评估了氨基甲酸二乙酯作为螯合剂对镉排泄的影响。本研究旨在表征氨基甲酸二乙酯作为口服药物螯合剂在施用镉 60 天后的潜在功效。这种螯合剂显着增强了镉的尿液和胆汁排泄，并恢复了改变后的铁水平。给予镉后立即给不同组的大鼠口服氨基甲酸二乙酯，为期 1 周。螯合治疗后，通过腹主动脉放血处死动物。收集并制备血液、肾脏、脾脏和心脏样本以测定镉。分别采用石墨炉法和火焰原子吸收光谱法（GFAAS 和 FAAS）方法测定不同样品中的镉和铁浓度。螯合治疗结果显示，氨基甲酸二乙酯能够去除不同组织中的镉离子，同时铁浓度恢复到正常水平，临床症状也减轻。综上所述，氨基甲酸二乙酯可以动员和促进大鼠器官中镉的排泄，减轻毒副作用和一般毒性症状。此外，它可能有助于初步测试螯合剂在人体内的效率。然而，这些结果应该在外推到其他系统之前在不同的实验模型中得到确认。当然，该测试程序并不能提供评估螯合剂镉毒性效率的所有相关答案。
The effect of diethyl carbamate as a chelating agent on the excretion of cadmium was evaluated in cadmium-poisoned Wistar rats following food and drink administration. The present research aimed to characterize the potential efficiency of diethyl carbamate as an oral drug chelator after cadmium administration for 60 days. This chelator significantly enhanced the urinary and biliary excretion of cadmium and restored the altered levels of iron. Diethyl carbamate was given orally to different groups of rats for a period of 1 week immediately after cadmium administration. After chelation therapy, animals were killed by exsanguination from abdominal aorta. Blood, kidneys, spleen, and heart samples were collected and prepared for determination of cadmium. Cadmium and iron concentrations in different issues were determined by graphite furnace and flame atomic absorption spectrometry (GFAAS and FAAS) methods, respectively. The chelation therapy results showed that diethyl carbamate was able to remove cadmium ions from different tissues while iron concentration returned to normal level and clinical symptoms were also reduced. In summary, we can conclude that diethyl carbamate could mobilize and promote the excretion of cadmium in rat organs and reduce the side-effects and general symptoms of toxicity. Moreover, it might be useful for preliminary testing of the efficiency of chelating agents in the human body. However, these results should be confirmed in a different experimental model before extrapolation to other systems. This testing procedure of course does not provide all the relevant answers for evaluating the efficiency of chelating agents in cadmium toxicity.