HERPES-SIMPLEX VIRUS VP16 FORMS A COMPLEX WITH THE VIRION HOST SHUTOFF PROTEIN VHS
HERPES-SIMPLEX VIRUS VP16 FORMS A COMPLEX WITH THE VIRION HOST SHUTOFF PROTEIN VHS
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DOI：
10.1128/jvi.68.4.2339-2346.1994
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发表时间：
1994-04-01
影响因子：
5.4
通讯作者：
SMILEY, JR
中科院分区：
文献类型：
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作者：
SMIBERT, CA;POPOVA, B;SMILEY, JR
单纯疱疹病毒 (HSV) 病毒颗粒含有至少两种调节受感染细胞中基因表达的调节蛋白：转录激活剂 VP16 和病毒颗粒宿主关闭蛋白 vhs。 VP16 刺激 HSV 立即早期基因的转录，而 vhs 抑制宿主蛋白质合成并诱导细胞和病毒 mRNA 的加速周转。我们在此报告，vhs 直接与 VP16 结合：vhs 和 VP16 通过抗 vhs 抗血清从感染细胞中共沉淀，并且在固相捕获测定中，vhs 和 VP16 蛋白 A 融合体各自结合另一种蛋白的完整版本。此外，当在酿酒酵母中共表达时，vhs和VP16在双杂交激活剂系统中相互作用。 vhs 残基 238 至 344 足以相互作用，并且不需要 VP16 酸性转录激活结构域。 vhs 阻断了 VP16 进入立即早期 TAATGARATTC 共有序列上的多蛋白复合物的能力，表明 vhs 与启动子识别所需的 VP16 的一个或多个区域相互作用。我们认为这种相互作用可能在 HSV 病毒颗粒的组装中发挥结构作用，并在感染过程中调节 vhs 的活性。
Herpes simplex virus (HSV) virions contain at least two regulatory proteins that modulate gene expression in infected cells: the transcriptional activator VP16 and the virion host shutoff protein vhs. VP16 stimulates transcription of the HSV immediate early genes, and vhs suppresses host protein synthesis and induces accelerated turnover of cellular and viral mRNAs. We report here that vhs binds directly to VP16: vhs and VP16 were coprecipitated from infected cells by an anti-vhs antiserum, and vhs and VP16 protein A fusions each bound intact versions of the other protein in a solid-phase capture assay. In addition, vhs and VP16 interacted in the two-hybrid activator system when coexpressed in Saccharomyces cerevisiae. vhs residues 238 to 344 were sufficient for the interaction, and the VP16 acidic transcriptional activation domain was not required. vhs blocked the ability of VP16 to enter a multiprotein complex on an immediate-early TAATGARATTC consensus sequence, indicating that vhs interacts with one or more regions of VP16 required for promoter recognition. We suggest that this interaction may play a structural role in the assembly of HSV virions and modulate the activity of vhs during infection.