A P-type ATPase required for rice blast disease and induction of host resistance
A P-type ATPase required for rice blast disease and induction of host resistance
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DOI：
10.1038/nature04567
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发表时间：
2006-03-23
期刊：
影响因子：
64.8
通讯作者：
Talbot, NJ
中科院分区：
文献类型：
--
作者：
Gilbert, MJ;Thornton, CR;Talbot, NJ
为了在植物中引起疾病，病原微生物已经进化出将蛋白质直接运送到植物细胞中的机制，在那里它们抑制植物防御并促进组织入侵(1-3)。导致世界上许多最严重的植物疾病的植物病原真菌在植物感染期间如何运送蛋白质目前尚不清楚。在这里，我们报道了经济上重要的稻瘟病菌中P型ATPase编码基因mGAPT2的特征，该基因在植物侵染过程中是胞吐所必需的。靶向基因替换表明，该真菌的叶部和根部感染以及在不亲和反应中快速诱导宿主防御反应都需要mGAPT2。Delta Mgapt2突变体在一系列胞外酶的分泌方面受到损害，并积累异常的高尔基体样脑池。然而，MgAPT2的缺失对菌丝生长或产孢量没有显著影响，这表明稻瘟病的建立涉及依赖于MgApt2的胞吐过程的使用，这种过程在植物侵染过程中运行。
To cause diseases in plants, pathogenic microorganisms have evolved mechanisms to deliver proteins directly into plant cells, where they suppress plant defences and facilitate tissue invasion(1-3). How plant pathogenic fungi, which cause many of the world's most serious plant diseases, deliver proteins during plant infection is currently unknown. Here we report the characterization of a P-type ATPase-encoding gene, MgAPT2, in the economically important rice blast pathogen Magnaporthe grisea, which is required for exocytosis during plant infection. Targeted gene replacement showed that MgAPT2 is required for both foliar and root infection by the fungus, and for the rapid induction of host defence responses in an incompatible reaction. Delta Mgapt2 mutants are impaired in the secretion of a range of extracellular enzymes and accumulate abnormal Golgi-like cisternae. However, the loss of MgAPT2 does not significantly affect hyphal growth or sporulation, indicating that the establishment of rice blast disease involves the use of MgApt2-dependent exocytotic processes that operate during plant infection.