Induced G1 phase arrest of fast-dividing cells improves the quality of genomic profiles generated by array-CGH
Induced G1 phase arrest of fast-dividing cells improves the quality of genomic profiles generated by array-CGH
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DOI：
10.2144/0000113938
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发表时间：
2012-10-01
期刊：
影响因子：
2.7
通讯作者：
Steinemann, Doris
中科院分区：
文献类型：
--
作者：
Manukjan, Georgi;Tauscher, Marcel;Steinemann, Doris
通过基于阵列的高分辨率比较基因组杂交（array-CGH）对拷贝数变化进行全基因组谱分析是确保不同条件下细胞基因组完整性的重要方法。我们观察到基因组谱的分析，特别是快速分裂的小鼠白血病细胞系，是具有挑战性的，因为特征模式在阵列- cgh基线周围振荡。在这里，我们展示了阵列- cgh数据可以通过使用剥夺方案或细胞周期抑制剂降低培养细胞的增殖率大大提高。在G1期阻滞细胞周期导致更平滑的基因组图谱，从而更可靠地检测拷贝数改变。
Genome-wide profiling of copy number alterations by array-based high resolution comparative genomic hybridization (array-CGH) is an important method to ensure the genomic integrity of cells in diverse conditions. We observed that the analysis of genomic profiles, in particular of fast-dividing murine leukemia cell lines, is challenging due to characteristic patterns oscillating around the array-CGH baseline. Here we show array-CGH data can be drastically improved by reducing proliferation rates of cultured cells using deprivation protocols or cell cycle inhibitors. Arresting cell cycle in the G1 phase leads to smoother genomic profiles, and hence to a more reliable detection of copy number alterations.