Demonstration of nanosecond dipolar relaxation in biopolymers by inversion of apparent fluorescence phase shift and demodulation lifetimes.
Demonstration of nanosecond dipolar relaxation in biopolymers by inversion of apparent fluorescence phase shift and demodulation lifetimes.
复制标题
通过表观荧光相移和解调寿命的反转来演示生物聚合物中的纳秒偶极弛豫。
DOI：
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发表时间：
1980
影响因子：
4.8
通讯作者：
D. Bevan
中科院分区：
文献类型：
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作者：
J. Lakowicz;H. Cherek;D. Bevan
荧光相移和解调方法不能直接揭示样品的衰减规律。因此，这些方法通常不用于研究溶剂、蛋白质和膜中的偶极弛豫。我们报道了荧光发射时间尺度上的光谱弛豫导致表观调制寿命（T…）小于表观相位寿命（T+），即T…
Fluorescence phase shift and demodulation methods do not directly reveal the decay law of the sample. As a result, these methods are generally not used to investigate the dipolar relaxation in solvents, proteins, and membranes. We report that spectral relaxation on the time scale of the fluorescence emission results in apparent modulation lifetimes (T,,,) which are smaller than the apparent phase lifetimes (T+), that is T,,,