NaoXinTong Inhibits the Development of Diabetic Retinopathy in db/db Mice
NaoXinTong Inhibits the Development of Diabetic Retinopathy in db/db Mice
复制标题
脑心通抑制 db/db 小鼠糖尿病视网膜病变的发展。
DOI：
10.1155/2015/242517
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Duan, Yajun
中科院分区：
文献类型：
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作者：
Liu, Mengyang;Pan, Quan;Duan, Yajun
步长脑心通胶囊（NXT）是从16味中草药中提取的中药标准化产品，在我国广泛用于治疗心脑血管疾病。微动脉瘤的形成在糖尿病视网膜病变的发展中起重要作用。在这项研究中，我们研究了NXT是否可以保护糖尿病小鼠免受糖尿病视网膜病变的发展。将db/db小鼠（与6周龄相似）（糖尿病动物模型）分为两组，分别饲喂正常饲料或加NXT 14周。治疗期间每月测定空腹血糖水平。处理后，收集视网膜以测定视网膜厚度、碳水化合物大分子的积累和半胱天冬酶-3（CAS-3）表达。我们的研究结果表明，NXT的管理降低空腹血糖水平。NXT可阻断糖尿病引起的视网膜光感受器层、外核/丛状层等多个层的萎缩，并伴有葡萄糖水平的降低。NXT还抑制糖尿病诱导的CAS-3蛋白和mRNA、MMP-2/9和TNF α mRNA的表达、碳水化合物大分子的积累以及视网膜中无细胞毛细血管的形成。综上所述，我们的研究表明，NXT可以抑制糖尿病视网膜病变的发展，并提出了一个新的潜在的临床应用NXT。
Buchang NaoXinTong capsule (NXT) is a Chinese Materia Medica standardized product extracted from 16 Chinese traditional medical herbs and widely used for treatment of patients with cerebrovascular and cardiovascular diseases in China. Formation of microaneurysms plays an important role in the development of diabetic retinopathy. In this study, we investigated if NXT can protect diabetic mice against the development of diabetic retinopathy. The db/db mice (similar to 6 weeks old), a diabetic animal model, were divided into two groups and fed normal chow or plus NXT for 14 weeks. During the treatment, fasting blood glucose levels were monthly determined. After treatment, retinas were collected to determine retinal thickness, accumulation of carbohydrate macromolecules, and caspase-3 (CAS-3) expression. Our results demonstrate that administration of NXT decreased fasting blood glucose levels. Associated with the decreased glucose levels, NXT blocked the diabetes-induced shrink of multiple layers, such as photoreceptor layer and outer nuclear/plexiform layers, in the retina. NXT also inhibited the diabetes-induced expression of CAS-3 protein and mRNA, MMP-2/9 and TNF alpha mRNA, accumulation of carbohydrate macromolecules, and formation of acellular capillaries in the retina. Taken together, our study shows that NXT can inhibit the development of diabetic retinopathy and suggests a new potential application of NXT in clinic.