In Vitro Antiinflammatory Activity of a New Sesquiterpene Lactone Isolated from Siegesbeckia glabrescens
In Vitro Antiinflammatory Activity of a New Sesquiterpene Lactone Isolated from Siegesbeckia glabrescens
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DOI：
10.1002/ptr.3420
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发表时间：
2011-09-01
影响因子：
7.2
通讯作者：
Ryu, Jae-Ha
中科院分区：
文献类型：
--
作者：
Li, Hua;Kim, Jae Yeon;Ryu, Jae-Ha
在lps激活的RAW264.7巨噬细胞中分离到一种新的倍半萜内酯，可抑制一氧化氮和前列腺素E-2的合成。剂量依赖性地降低了诱导型一氧化氮合酶和环氧合酶-2的蛋白和mRNA水平。报告基因实验显示，该化合物可减弱lps诱导的核因子κ B （nf - κ B）的DNA转录活性。此外，它通过阻断抑制kappa B- α降解，抑制NF-kappa B p65亚基的核易位。该化合物可能对炎症相关疾病的治疗有益。版权所有：John Wiley & Sons， Ltd。
A new sesquiterpene lactone was isolated from Siegesbeckia glabrescens as an inhibitor of nitric oxide and prostaglandin E-2 synthesis in the LPS-activated RAW264.7 macrophage cell line. It dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase and cyclooxygenase-2. Reporter gene assay revealed that the compound attenuated the LPS-induced DNA transcriptional activity of nuclear factor-kappa B (NF-kappa B). In addition, it inhibited the nuclear translocation of the p65 subunit of NF-kappa B by blocking the inhibitory kappa B-alpha degradation. This compound from Siegesbeckia glabrescens might be beneficial for the treatment of inflammation-related diseases. Copyright (C) 2011 John Wiley & Sons, Ltd.