VENTRAL LATERAL GENICULATE-NUCLEUS EFFERENTS TO THE RAT SUPRACHIASMATIC NUCLEUS EXHIBIT AVIAN PANCREATIC POLYPEPTIDE-LIKE IMMUNOREACTIVITY
VENTRAL LATERAL GENICULATE-NUCLEUS EFFERENTS TO THE RAT SUPRACHIASMATIC NUCLEUS EXHIBIT AVIAN PANCREATIC POLYPEPTIDE-LIKE IMMUNOREACTIVITY
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DOI：
10.1002/cne.902060407
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发表时间：
1982-01-01
影响因子：
2.5
通讯作者：
MOORE, RY
中科院分区：
文献类型：
--
作者：
CARD, JP;MOORE, RY
用免疫组织化学方法研究了大鼠下丘脑视交叉上核(SCN)内鸟胰腺多肽样(APP)免疫反应的分布。用纯APP抗原预吸附的抗血清孵育的组织中没有所有免疫反应染色的组织，从而确定了抗血清的特异性。此外，抗血清与血管活性肠肽、加压素、生长抑素或促胰液素没有明显的交叉反应。在大鼠脊髓束核内，APP免疫反应仅限于核腹侧和外侧的曲张轴突；核背内侧部分完全没有免疫反应，SCN的任何部分都没有核周膜。核腹外侧部的免疫反应阳性轴突形成广泛的神经丛，其分布与视网膜和腹侧膝状核(VLGN)传出至SCN的分布密切相关。APP免疫过氧化物酶染色后，在视网膜内没有观察到免疫反应阳性的核周膜，单侧和双侧摘除都没有引起SCN内APP轴突分布模式的明显改变，从而表明该纤维丛不是视网膜起源的。APP免疫反应阳性神经元出现在vLGN的同一区域，在该区域，向SCN注入HRP后，已发现逆行填充的神经元。双侧vLGN的电解性损害导致双侧SCN内免疫反应阳性轴突的完全丧失。单侧vLBN损伤会导致.apprx的丢失。60%~75%的免疫反应纤维位于同侧SCN内，对侧损伤较少。这些观察结果进一步提供了有关大鼠SCN的传入组织的信息，并表明vLGN投射在化学上与其他SCN传入不同。
The distribution of avian pancreatic polypeptide-like (APP) immunoreactivity was investigated in the suprachiasmatic nucleus (SCN) of the rat hypothalamus with immunohistochemical methods. Specificity of the antisera was established by the absence of all immunoreactive staining in tissue incubated in antisera which had been preabsorbed with the pure APP antigen. In addition, the antisera exhibited no significant cross reactivity with vasoactive intestinal polypeptide, vasopressin, somatostatin, or secretin. Within the rat SCN, APP immunoreactivity is restricted to varicose axons in the ventral and lateral aspects of the nucleus; the dorsomedial component of the nucleus is totally devoid of immunoreactivity and immunoreactive perikarya are not present in any portion of the SCN. The immunoreactive axons in the ventrolateral portion of the nucleus form an extensive plexus which is distributed in a pattern closely corresponding to the distribution of retinal and ventral lateral geniculate (vLGN) efferents to the SCN. No immunoreactive perikarya are observed in the retina following immunoperoxidase staining for APP and neither unilateral nor bilateral enucleation causes an observable alteration in the pattern of APP axon distribution within the SCN, thus indicating that the fiber plexus is not of retinal origin. APP immunoreactive neurons are present in the same area of the vLGN in which retrogradely filled neurons have been demonstrated following iontophoretic injection of HRP [horseradish peroxidase] into the SCN. Bilateral electrolytic lesions of the vLGN rsult in a total loss of immunoreactive axons in both SCN. Unilateral vLBN lesions cause a loss of .apprx. 60-75% of the immunoreactive fibers in the ipsilateral SCN with a lesser contralateral loss. These observations provide further information on the organization of afferents to the rat SCN and demonstrate that the vLGN projection is chemically distinct from other SCN afferents.