KINETICS OF ANTIGEN SPECIFIC AND NONSPECIFIC POLYCLONAL B-CELL RESPONSES DURING LETHAL PLASMODIUM-YOELII MALARIA
KINETICS OF ANTIGEN SPECIFIC AND NONSPECIFIC POLYCLONAL B-CELL RESPONSES DURING LETHAL PLASMODIUM-YOELII MALARIA
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DOI：
10.1590/s0074-02761992000200005
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发表时间：
1992-04-01
影响因子：
2.8
通讯作者：
DANIELRIBEIRO, C
中科院分区：
文献类型：
--
作者：
ROLLAND, L;BALLET, JJ;DANIELRIBEIRO, C
为了研究与疟疾感染相关的多克隆B细胞活化的动力学和组成，在感染约氏疟原虫17 XL或注射来自约氏疟原虫感染小鼠的裂解红细胞或血浆或注射恶性疟原虫培养物上清液的小鼠的脾脏中评价抗原特异性和非特异性B细胞应答。脾/体重比、有核脾细胞和含免疫球蛋白和免疫球蛋白分泌细胞的数量在感染过程中进行性增加，与寄生虫血症平行。在研究抗绵羊红细胞和抗三硝基苯基化绵羊红细胞空斑形成细胞反应时，观察到不同的动力学模式：在感染的早期阶段观察到最大值，而含有免疫球蛋白和免疫球蛋白分泌细胞的总数尚未改变。相反，在感染结束时，当后者的值达到其最大值时，抗绵羊红细胞和抗三硝基苯基化绵羊红细胞特异性反应是正常的，甚至是超常的。在注射疟原虫衍生材料的小鼠中，与含免疫球蛋白和分泌免疫球蛋白的细胞数量的增加相比，观察到抗原特异性PFC的增加更高。这表明抗原特异性细胞的“优先”（抗原加有丝分裂原诱导的）刺激，而不是B淋巴细胞的一般非特异性（有丝分裂原诱导的）触发。在这些和以前的结果的基础上，它表明，在感染过程中发生的多克隆B细胞活化出现作为连续波的抗原特异性B细胞活化的结果。
In order to study the kinetics and composition of the polyclonal B-cell activation associated to malaria infection, antigen-specific and non-specific B-cell responses were evaluated in the spleens of mice infected with Plasmodium yoelii 17XL or injected with lysed erythrocytes or plasma from P. yoelii infected mice or with P. falciparum culture supernatants. Spleen/body weight ratio, numbers of nucleated spleen cells and Immunoglobulin-containing and Immunoglobulin-secreting cells increased progressively during the course of infection, in parallel to the parasitaemia. A different pattern of kinetics was observed when anti-sheep red blood cell and anti-trinitrophenylated-sheep red blood cell plaque forming cells response were studied: maximum values were observed at early stages of infection, whereas the number of total Immunoglobulin-containing and Immunoglobulin-secreting cells were not yet altered. Conversely, at the end of infection, when these latter values reached their maximum, the anti-sheep red blood cell and anti-trinitrophenylated-sheep red blood cell specific responses were normal or even infranormal. In mice injected with Plasmodium-derived material, a higher increase in antigen-specific PFC was observed, as compared to the increase of Immunoglobulin-containing and Immunoglobulin-secreting cell numbers. This suggested a "preferential" (antigen-plus mitogen-induced) stimulation of antigen-specific cells rather than a generalized non-specific (mitogen-induced) triggering of B-lymphocytes. On the basis of these and previous results, it is suggested that the polyclonal B-cell activation that takes place during the course of infection appears as a result of successive waves of antigen-specific B-cell activation.