Parathyroid hormone-related peptide (1-34) promotes tooth eruption and inhibits osteogenesis of dental follicle cells during tooth development.
Parathyroid hormone-related peptide (1-34) promotes tooth eruption and inhibits osteogenesis of dental follicle cells during tooth development.
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甲状旁腺激素相关肽（1-34）在牙齿发育过程中促进牙齿萌出并抑制牙囊细胞成骨
DOI：
10.1002/jcp.27857
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Shujuan Zou
中科院分区：
文献类型：
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作者：
Jiayu Zhang;Lijun Liao;Yuyu Li;Yang Xu;Weihua Guo;Weidong Tian;Shujuan Zou
牙囊细胞（DFC）激活并募集破骨细胞用于牙齿发育和牙齿萌出，而DFC本身通过与Hertwig上皮根鞘（HERS）相互作用分化成骨细胞以形成牙根周围的牙槽骨。此外，在牙齿发育过程中，甲状旁腺激素相关肽（PTHrP）在牙胚周围表达。因此，我们的目的是研究PTHrP（1-34）在体外和体内对DFCs骨吸收和成骨的影响。体外研究表明，与HERS细胞共培养的DFCs表达的BSP和OPN水平高于DFCs对照组，而与共培养的DFCs对照组相比，PTHrP（1-34）处理的DFCs表达的ALP、RUNX 2、BSP和OPN水平较低。此外，我们发现PTHrP（1-34）通过失活Wnt/β-catenin途径抑制共培养的DFC的成骨。PTHrP（1-34）还可增加RANKL/OPG比值的表达。与此同时，体内实验发现PTHrP（1-34）促进牙齿萌出，抑制牙槽骨形成。因此，这些结果表明PTHrP（1-34）通过使Wnt/β-catenin通路失活而促进牙齿萌出并抑制DFC的成骨。
Dental follicle cells (DFCs) activate and recruit osteoclasts for tooth development and tooth eruption, whereas DFCs themselves differentiate into osteoblasts to form alveolar bone surrounding tooth roots through the interaction with Hertwig's epithelial root sheath (HERS). Also during tooth development, parathyroid hormone‐related peptide (PTHrP) is expressed surrounding the tooth germ. Thus, we aimed to investigate the effect of PTHrP (1–34) on bone resorption and osteogenesis of DFCs in vitro and in vivo. In vitro studies demonstrated that DFCs cocultured with HERS cells expressed higher levels of BSP and OPN than the DFCs control group, whereas cocultured DFCs treated with PTHrP (1–34) had lower expressions of ALP, RUNX2, BSP, and OPN than the cocultured DFCs control group. Moreover, we found PTHrP (1–34) inhibited osteogenesis of cocultured DFCs by inactivating the Wnt/β‐catenin pathway. PTHrP (1–34) also increased the expression of RANKL/OPG ratio in DFCs. Consistently, in vivo study found that PTHrP (1–34) accelerated tooth eruption and inhibited alveolar bone formation. Therefore, these results suggest that PTHrP (1–34) accelerates tooth eruption and inhibits osteogenesis of DFCs by inactivating Wnt/β‐catenin pathway.