TRANSFORMATION OF MYCOPLASMA-PULMONIS AND MYCOPLASMA-HYORHINIS - TRANSPOSITION OF TN916 AND FORMATION OF COINTEGRATE STRUCTURES
TRANSFORMATION OF MYCOPLASMA-PULMONIS AND MYCOPLASMA-HYORHINIS - TRANSPOSITION OF TN916 AND FORMATION OF COINTEGRATE STRUCTURES
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DOI：
10.1016/0147-619x(88)90005-4
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发表时间：
1988-07-01
期刊：
影响因子：
2.6
通讯作者：
ALDERETE, J
中科院分区：
文献类型：
--
作者：
DYBVIG, K;ALDERETE, J
建立了用质粒PAM 120转化小鼠肺支原体的方法。该质粒在大肠杆菌中复制，含有革兰氏阳性转座子Tn916。该转化方案对猪鼻支原体也被证明是有效的。在转化的支原体细胞中表达了Tn916的四环素抗性决定簇，并发现Tn916插入了肺支原体和猪鼻支原体受体染色体的多个位置，表明发生了转座。有趣的是，一些转化的肺分枝杆菌和猪分枝杆菌含有共整合结构，这种结构显然将整个供体质粒(PAM120)的完整拷贝插入到受体染色体中。随后在转化的肺支原体传代克隆中观察到插入的Tn916的转位。
A procedure for transformation of the murine pathogen Mycoplasma pulmonis with plasmid pAM 120 was developed. This plasmid replicates in Escherichia coli and contains the gram-positive transposon Tn916. The transformation protocol also proved effective for the swine pathogen Mycoplasma hyorhinis. The tetracycline resistance determinant of Tn916 was expressed in transformed mycoplasma cells, and Tn916 was found inserted into numberous sites in the recipient chromosomes of M. pulmonis and M. hyorhinis, indicating that transposition had occurred. Interestingly, some transformation of M. pulmonis and M. hyorhinis contained cointegrate structures which apparently had a complete copy of the entire donor plasmid (pAM120) inserted into the recipient chromosome. Subsequent transposition of inserted Tn916 was observed in passaged clones of transformed M. pulmonis.