A Survey of the ATP-Binding Cassette (ABC) Gene Superfamily in the Salmon Louse (Lepeophtheirus salmonis).
A Survey of the ATP-Binding Cassette (ABC) Gene Superfamily in the Salmon Louse (Lepeophtheirus salmonis).
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DOI：
10.1371/journal.pone.0137394
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Sturm A
中科院分区：
文献类型：
--
作者：
Carmona-Antoñanzas G;Carmichael SN;Heumann J;Taggart JB;Gharbi K;Bron JE;Bekaert M;Sturm A
鲑鱼虱（Lepeophtheirus salmonis，Krøyer，1837）是鱼类体外寄生虫，在大西洋鲑鱼（萨尔莫salar Linnaeus，1758）的海水养殖中造成重大经济损失。L.养鱼场的鲑鱼在很大程度上依赖于抗寄生虫药物的治疗。与化学防治相关的一个问题是抗性的发展潜力，在L.据记载，鲑鱼可用于多种药物类别，包括有机磷酸盐、拟除虫菊酯和阿维菌素。ATP结合盒（ABC）基因超家族存在于所有生物群中，包括一系列可赋予癌症和病原体耐药性的药物外排转运蛋白。此外，一些ABC转运蛋白被认为参与了杀虫剂抗性的赋予。虽然许多研究已经研究了L. salmonis，没有系统的分析ABC基因家族存在这个物种。本研究提出了一个全基因组调查的ABC基因在L。salmonis，其中，ABC超家族成员通过L.鲑鱼基因组此外，在通过多阶段RNA文库的高通量RNA测序（RNA-seq）产生的寄生虫的参考转录组中鉴定ABC蛋白。基因组和转录组的测序允许鉴定总共33个编码ABC蛋白的基因/转录物，其中3个仅在基因组中表示，4个仅在转录组中表示。18个序列被分配到已知含有药物转运蛋白的ABC亚家族，即亚家族B（4个序列）、C（11个）和G（2个）。结果表明，L.鲑属的成员比记录的其他节肢动物少。本文介绍了我国北方地区L.鲑鱼ABC基因超家族的建立将为进一步研究ABC转运蛋白在鲑鱼灭虱剂毒性中的潜在作用以及作为鲑鱼抗药性的潜在机制提供基础。
Salmon lice, Lepeophtheirus salmonis (Krøyer, 1837), are fish ectoparasites causing significant economic damage in the mariculture of Atlantic salmon, Salmo salar Linnaeus, 1758. The control of L. salmonis at fish farms relies to a large extent on treatment with anti-parasitic drugs. A problem related to chemical control is the potential for development of resistance, which in L. salmonis is documented for a number of drug classes including organophosphates, pyrethroids and avermectins. The ATP-binding cassette (ABC) gene superfamily is found in all biota and includes a range of drug efflux transporters that can confer drug resistance to cancers and pathogens. Furthermore, some ABC transporters are recognised to be involved in conferral of insecticide resistance. While a number of studies have investigated ABC transporters in L. salmonis, no systematic analysis of the ABC gene family exists for this species. This study presents a genome-wide survey of ABC genes in L. salmonis for which, ABC superfamily members were identified through homology searching of the L. salmonis genome. In addition, ABC proteins were identified in a reference transcriptome of the parasite generated by high-throughput RNA sequencing (RNA-seq) of a multi-stage RNA library. Searches of both genome and transcriptome allowed the identification of a total of 33 genes / transcripts coding for ABC proteins, of which 3 were represented only in the genome and 4 only in the transcriptome. Eighteen sequences were assigned to ABC subfamilies known to contain drug transporters, i.e. subfamilies B (4 sequences), C (11) and G (2). The results suggest that the ABC gene family of L. salmonis possesses fewer members than recorded for other arthropods. The present survey of the L. salmonis ABC gene superfamily will provide the basis for further research into potential roles of ABC transporters in the toxicity of salmon delousing agents and as potential mechanisms of drug resistance.