Unloading induces transcriptional activation of the sarco(endo)plasmic reticulum Ca2+-ATPase 1 gene in muscle.
Unloading induces transcriptional activation of the sarco(endo)plasmic reticulum Ca2+-ATPase 1 gene in muscle.
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卸载诱导肌肉中肌（内）质网 Ca2 -ATPase 1 基因的转录激活。
DOI：
10.1152/ajpcell.1999.276.5.c1218
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Kandarian,SC
中科院分区：
文献类型：
--
作者：
Peters,DG;Mitchell-Felton,H;Kandarian,SC
先前的研究表明，在无负荷的慢收缩比目鱼肌中，sarco（endo）质网Ca2+- atp酶（SERCA1）的“快速”异构体的蛋白质和mRNA水平显着增加，提示翻译前控制基因表达[L]。M.舒尔特，J.纳瓦罗和S. C.坎达里安。[j].中国生物医学工程学报，2016,32(2):387 - 398。然而，由于难以测量整个肌肉的转录率，SERCA1基因的转录激活与卸载尚未得到证实。由于SERCA1前mrna水平可以反映转录活性，因此本研究对SERCA1内含子进行测序，以允许内含子定向RT-PCR测量SERCA1前mrna。然后将这些数据与对照和无负荷比目鱼肌中SERCA1 mRNA表达的变化进行比较。卸载2、4和10 d后，SERCA1 pre-mRNA和mRNA转录物水平分别显著增加了2倍、3倍和7倍（P< 0.01）。SERCA1 pre-mRNA和mRNA的平行增加表明内源性SERCA1基因通过肌肉卸载被转录激活。SERCA2，心脏/慢抽搐骨骼肌同种型，卸载后没有明显增加，RNase保护试验显示SERCA1或SERCA2初级转录物的选择性剪接没有变化。使用体内质粒注射，由3.6 kb SERCA1 5 ' -侧翼区域驱动的报告基因的活性在7天无负荷的比目鱼肌肉中增加了5倍。通过卸载内源性和构建的SERCA1基因转录激活程度的比较证实了使用内含子RT-PCR检测肌肉基因转录率的准确性，并表明该启动子结构中包含足以调节卸载诱导的转录激活的顺式作用元件。
Previous work showed that protein and mRNA levels of the “fast” isoform of the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA1) are markedly increased in unloaded slow-twitch soleus muscles, suggesting pretranslational control of gene expression [L. M. Schulte, J. Navarro, and S. C. Kandarian.Am. J. Physiol.264 (Cell Physiol.33): C1308–C1315, 1993]. However, because of the difficulty of measuring transcription rates from whole muscle, transcriptional activation of the SERCA1 gene with unloading has not been confirmed. Because SERCA1 pre-mRNA levels can reflect transcriptional activity, in the present study SERCA1 introns were sequenced to allow intron-directed RT-PCR measurement of SERCA1 pre-mRNA. These data were then compared with changes in SERCA1 mRNA expression in control and unloaded soleus muscles. After 2, 4, and 10 days of unloading, SERCA1 pre-mRNA and mRNA transcript levels increased significantly by two-, three-, and sevenfold, respectively (P< 0.01). Parallel increases in SERCA1 pre-mRNA and mRNA suggest transcriptional activation of the endogenous SERCA1 gene by muscle unloading. SERCA2, the cardiac/slow-twitch skeletal muscle isoform, was not markedly increased by unloading, and RNase protection assays showed no change in alternative splicing of SERCA1 or SERCA2 primary transcripts. With use of in vivo plasmid injection, the activity of a reporter gene driven by 3.6 kb of the SERCA1 5′-flanking region increased fivefold in 7-day-unloaded soleus muscles. Comparison of the magnitude of transcriptional activation of endogenous and constructed SERCA1 genes by unloading confirms the fidelity of using intronic RT-PCR to examine muscle gene transcription rates and suggests thatcis-acting elements sufficient for regulating unloading-induced transcriptional activation are contained in this promoter construct.
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甲状腺激素和肌生成素转录调节之间的串扰：快速型肌浆网 Ca2+-ATP 酶的 Ca2+ 依赖性表达的新方面。
影响因子：
4.1
作者：
M. Thelen;W. Simonides;A. Muller;C. van Hardeveld
通讯作者：
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DOI：
10.1016/s0167-4781(97)00135-8
发表时间：
1998
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Hu,P;Zhang,KM;Spratt,JA;Wechsler,AS;Briggs,FN
通讯作者：
Briggs,FN
大鼠肌浆网 Ca2 腺苷三磷酸酶基因的转录通过 3,5,3-三碘甲状腺原氨酸受体异构体与肌细胞特异性增强因子 2a 的特异性相互作用而增加。
DOI：
10.1210/endo.138.1.4857
发表时间：
1997
期刊：
Endocrinology.
影响因子：
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作者：
Moriscot,AS;Sayen,MR;Hartong,R;Wu,P;Dillmann,WH
通讯作者：
Dillmann,WH
基因转移到肌肉。
影响因子：
3.9
作者：
G. Dickson
通讯作者：
G. Dickson
转基因小鼠悬浮和机械超载/悬浮比目鱼肌中的 β-MHC 转基因表达。
DOI：
10.1152/ajpregu.1997.272.5.r1552
发表时间：
1997
期刊：
The American journal of physiology.
影响因子：
--
作者：
McCarthy,JJ;Fox,AM;Tsika,GL;Gao,L;Tsika,RW
通讯作者：
Tsika,RW