Characterization of the production of acetaldehyde by astrocytes in culture after ethanol exposure
Characterization of the production of acetaldehyde by astrocytes in culture after ethanol exposure
复制标题
DOI：
10.1111/j.1530-0277.1997.tb04247.x
复制
发表时间：
1997-09-01
影响因子：
3.2
通讯作者：
Barret, L
中科院分区：
文献类型：
--
作者：
Eysseric, H;Gonthier, B;Barret, L
神经系统是乙醇中毒的主要靶点之一。星形胶质细胞可能在乙醇诱导的脑毒性中发挥重要作用，因为它们的完整性对神经元的正常生长和功能至关重要。另一方面，乙醛被认为是乙醇的一些生化、药理和行为效应的中介体。本研究旨在证明培养的星形胶质细胞从乙醇中产生乙醛的能力。在原代培养的星形胶质细胞中，乙醇的代谢和乙醛的产生是显著的。与通常在肝细胞中观察到的产量相比，这一产量相当低，但与在整个脑匀浆中测得的产量相同，并与生物活性水平相对应。这样的演示可能会为理解乙醇的神经毒性带来新的元素。
The nervous system is one of the main targets of ethanol toxicity. Astrocytes might play an important role in ethanol-induced brain toxicity, because their integrity is essential for the normal growth and functioning of neurons. On the other hand, acetaldehyde has been implicated as a mediator in some of the biochemical, pharmacological, and behavioral effects of ethanol. The present study aimed at demonstrating the ability of astrocytes in culture to produce acetaldehyde from ethanol. Significant metabolization of ethanol with production of acetaldehyde was demonstrated in the primary culture of astrocytes. This production was quite low, compared with that usually observed in hepatocytes, but was in the same range as that measured in whole brain homogenates and corresponded to biologically active levels. Such a demonstration could bring new elements for understanding of ethanol neurotoxicity.