Heterogeneity of Porphyromonas gingivalis strains in the induction of alveolar bone loss in mice
Heterogeneity of Porphyromonas gingivalis strains in the induction of alveolar bone loss in mice
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DOI：
10.1034/j.1399-302x.2000.150105.x
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发表时间：
2000-02-01
影响因子：
--
通讯作者：
Roopenian, DC
中科院分区：
文献类型：
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作者：
Baker, PJ;Dixon, M;Roopenian, DC
这些实验检查了无特定病原体的小鼠口服牙龈卟啉单胞菌的模型中的牙槽骨损失。牙槽骨丢失是由牙龈卟啉单胞菌特异性感染引起的，而不是其他环境抗原。需要用活牙龈卟啉单胞菌感染，因为用福尔马林杀死的牙龈卟啉单胞菌灌胃后没有显著的骨丢失。比较不同菌株的毒力。两种实验室菌株的细菌（ATCC 53977和W50）和突变株缺乏43 kDa的菌毛蛋白（菌株DPG3）诱导骨丢失。然而，牙龈卟啉单胞菌381不诱导骨丢失。有一个强大的免疫球蛋白G（IgG）抗体反应感染与每一个菌株，但显着的血清伊加反应，只有菌株381。这些研究表明，在具有背景口腔微生物菌群的小鼠中，牙龈卟啉单胞菌的特异性感染诱导骨丢失，并且细菌菌株变异在确定牙槽骨丢失是否会随之发生方面是重要的。
These experiments examine alveolar bone loss in a model in which specific pathogen-free mice are exposed orally with Porphyromonas gingivalis. Alveolar bone loss was induced as a result of a specific infection with P. gingivalis, rather than other environmental antigens. Infection with live P. gingivalis was required, as significant bone loss did not follow gavage with formalin-killed P. gingivalis. The virulence of different strains of P. gingivalis was compared. Two laboratory strains of the bacteria (ATCC 53977 and W50) and a mutant strain lacking the 43-kDa fimbrillin (strain DPG3) induced bone loss. P. gingivalis 381, however, did not induce bone loss. There was a strong immunoglobulin G (IgG) antibody response to infection with each strain but a significant serum IgA response only to strain 381. These studies show that in mice with a background oral microflora bone loss is induced by a specific infection with P. gingivalis and that bacterial strain variation is important in determining whether alveolar bone loss will ensue.