Regulation of flowering time in Arabidopsis by K homology domain proteins
Regulation of flowering time in Arabidopsis by K homology domain proteins
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DOI：
10.1073/pnas.0404552101
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发表时间：
2004-08-24
影响因子：
11.1
通讯作者：
Lin, CT
中科院分区：
文献类型：
--
作者：
Mockler, TC;Yu, XH;Lin, CT
拟南芥从营养生长到生殖发育的转变受多种花诱导途径的调控，包括光周期途径、自主途径、春化途径和激素途径。这些途径汇聚在一起，调节对花启动至关重要的一小部分基因的表达，不同的信号转导途径可以相互作用，控制开花的时间。花启动的一个重要调节因子是MADS-box转录因子FLC，它作为开花的负调控因子对内源信号和环境信号做出反应。在这篇报道中，我们描述了开花时间基因flk(Flowering Locus K Homology(KH)域)的研究，该基因编码一个具有三个KH结构域的假定的RNA结合蛋白。Flk突变导致开花延迟，但对光周期或春化反应没有显著影响。Flk的主要功能是抑制FLC的表达，尽管它也对与光周期途径相关的基因的表达有一定的影响。除Flk外，另外两个KH结构域基因的表达也受到Flk突变的轻微影响，这表明可能有不止一个KH结构域蛋白参与了拟南芥开花时间的调控。
The transition from vegetative growth to reproductive development in Arabidopsis is regulated by multiple floral induction pathways, including the photoperiodic, the autonomous, the vernalization, and the hormonal pathways. These pathways converge to regulate the expression of a small set of genes critical for floral initiation and different signal transduction pathways can interact to govern the time to flower. One important regulator of floral initiation is the MADS-box transcription factor FLC, which acts as a negative regulator of flowering in response to both endogenous and environmental signals. In this report, we describe a study of the flowering-time gene, FLK [flowering locus K homology (KH) domain) that encodes a putative RNA-binding protein with three KH domains. The flk mutations cause delayed flowering without a significant effect on the photoperiodic or vernalization responses. FLK functions primarily as a repressor of FLC expression, although it also modestly affects expression of genes associated with the photoperiodic pathway. In addition to FLK, the expression of two other KH domain genes are modestly affected by the flk mutation, suggesting a possible involvement of more than one KH domain protein in the regulation of flowering time in Arabidopsis.