Over-expression of SOCS-3 gene promotes IL-10 production by JEG-3 trophoblast cells.
Over-expression of SOCS-3 gene promotes IL-10 production by JEG-3 trophoblast cells.
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DOI：
10.1016/j.placenta.2008.10.008
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发表时间：
2009-01
期刊：
影响因子：
3.8
通讯作者：
Wang, Y.
中科院分区：
文献类型：
--
作者：
Dong, Q.;Fan, R.;Zhao, S.;Wang, Y.
细胞因子信号转导抑制因子-3（SOCS-3）在炎症反应的负调控中起重要作用。有证据表明，SOCS-3和IL-10的表达显着降低胎盘滋养细胞从先兆子痫。IL-10是一种抗炎细胞因子。在这项研究中，我们试图确定增加SOCS-3表达是否会影响胎盘滋养层细胞产生IL-10。使用胎盘JEG-3细胞。通过siPORT脂质转染用绿色荧光蛋白（GFP）标记的SOCS-3基因SOCS-3/ZsGreen 1转染JEG-3细胞来产生SOCS-3的过表达。仅用ZsGreen 1载体转染的细胞用作对照。我们的结果表明，在用SOCS-3过表达的细胞中，IL-6的产生减少。此外，当用IL-6刺激时，SOCS-3转染的细胞产生更多的IL-10。JEG-3细胞产生IL-10的增加呈剂量依赖性，p < 0.05。我们的数据表明，增强SOCS-3基因表达可以促进胎盘滋养层细胞产生IL-10，表明SOCS-3可能在调节细胞因子诱导的胎盘滋养层细胞的抗炎反应中发挥重要作用。
Suppressor of cytokine signaling-3 (SOCS-3) plays an important role in negative regulation of inflammatory response. Evidence has shown that SOCS-3 and IL-10 expressions were significantly reduced in placental trophoblasts from preeclampsia. IL-10 is an anti-inflammatory cytokine. In this study, we sought to determine if enhance SOCS-3 expression could affect IL-10 production in placental trophoblasts. Placental JEG-3 cells were used. Over-expression of SOCS-3 was generated by transfection of JEG-3 cells with a green fluorescent protein (GFP) tagged SOCS-3 gene, SOCS-3/ZsGreen1, by siPORT lipid transfection. Cells transfected with ZsGreen1 vector only was used as control. Our results showed that IL-6 production was reduced in cells over-expressed with SOCS-3. Moreover, SOCS-3 transfected cells produced more IL-10 when stimulated with IL-6. The increased IL-10 production by JEG-3 cells was in a dose-dependent manner, p < 0.05. Our data suggested that enhanced SOCS-3 gene expression could promote IL-10 production by placental trophoblast cells, suggesting that SOCS-3 may play an important role in regulation of cytokine induced anti-inflammatory response in placental trophoblasts.
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影响因子：
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DOI：
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