A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins
A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins
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DOI：
10.1111/j.1365-2443.2007.01051.x
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发表时间：
2007-03-01
期刊：
影响因子：
2.1
通讯作者：
Fukui, Kiichi
中科院分区：
文献类型：
--
作者：
Takata, Hideaki;Uchiyama, Susumu;Fukui, Kiichi
比较蛋白质组分析人类中期染色体之间的一个典型的上皮样细胞，HeLa S3，和淋巴瘤型细胞，球1，进行。采用1-D SDS-PAGE和无自由基高还原性双向电泳（RFHR 2-DE）技术，从HeLa S3细胞染色体上分离到200多个蛋白质，其中189个蛋白质经质谱鉴定。与我们最近的四层结构模型的中期染色体相一致，所有确定的蛋白质被分为四个不同的丰度水平。HeLa S3和BALL-1染色体都含有丰富的染色体结构蛋白和外周蛋白，以及不太丰富的染色体包被蛋白（CCP）。此外，肌联蛋白阵列分析和超高分子量区域的蛋白质组分析表明不存在分子量（MW）大于1000 kDa的肌联蛋白。因此，目前的蛋白质组分析与以前的信息，染色体蛋白质提供了全面的列表中的蛋白质的中期染色体结构。
A comparative proteome analysis of human metaphase chromosomes between a typical epithelial-like cell, HeLa S3, and a lymphoma-type cell, BALL-1, was performed. One-dimensional (1-D) SDS-PAGE and radical-free and highly reducing two-dimensional electrophoresis (RFHR 2-DE) detected more than 200 proteins from chromosomes isolated from HeLa S3 cells, among which 189 proteins were identified by mass spectrometry (MS). Consistent with our recent four-layer structural model of a metaphase chromosome, all the identified proteins were grouped into four distinct levels of abundance. Both HeLa S3 and BALL-1 chromosomes contained specific sets of abundant chromosome structural and peripheral proteins in addition to less abundant chromosome coating proteins (CCPs). Furthermore, titin array analysis and a proteome analysis of the ultra-high molecular mass region indicated an absence of titin with their molecular weight (MW) more than 1000 kDa. Consequently, the present proteome analyses together with previous information on chromosome proteins provide the comprehensive list of proteins essential for the metaphase chromosome architecture.