Monoclonal antibody detection of laminin in minor salivary glands of patients with Sjögren's syndrome.
Monoclonal antibody detection of laminin in minor salivary glands of patients with Sjögren's syndrome.
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干燥综合征患者小唾液腺层粘连蛋白的单克隆抗体检测。
DOI：
10.1006/jaut.1993.1053
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发表时间：
1993
影响因子：
12.8
通讯作者：
P. Kragel
中科院分区：
文献类型：
--
作者：
C. McArthur;N. Fox;P. Kragel
通过比较基底膜蛋白、层粘连蛋白和纤连蛋白以及细胞骨架蛋白、微管蛋白在干燥综合征 (SS) 发病机制中的潜在作用，通过比较它们在 SS 与正常唇唾液腺 (LSG) 组织中的表达。使用过氧化物酶抗过氧化物酶技术中的充分表征的单克隆抗体，对来自 SS 患者和正常对照的福尔马林固定的 LSG 测定层粘连蛋白、纤连蛋白和微管蛋白的表达。层粘连蛋白的特征性导管周围染色发生在由一名观察者评分的 14/18 SS 患者的 LSG 中，以及由第二名观察者评分的 16/18 患者的 LSG 中。层粘连蛋白的 LSG 染色发生在 2/35 对照样本中，该对照样本由 15 个正常 LSG 和 20 个附有正常 LSG 的炎性病变组成。两个对照中发生的染色在一个病例中是弥漫性的且“非特异性”，并且与另一病例中在 SS 中发现的特征性导管周围染色无法区分。在 20 个含有炎性病变的对照中，有 4 个在实际病变内显示出层粘连蛋白的弥漫性染色，但相邻的 LSG 没有染色。抗纤连蛋白和抗微管蛋白染色的 SS 和正常组织之间没有观察到统计学上的显着差异。该研究得出的结论是，SS 患者唾液腺导管上皮上的层粘连蛋白或层粘连蛋白样物质有所增加。这表明层粘连蛋白在病理机制中的潜在作用，并可能表明层粘连蛋白表达增加是 SS 的标志。
The potential roles of the basement membrane proteins, laminin and fibronectin, and the cytoskeletal protein, tubulin, were assessed in the pathogenesis of Sjögren's Syndrome (SS) by comparing their expressions in SS with normal labial salivary gland (LSG) tissue. Laminin, fibronectin and tubulin expression were determined using well characterized monoclonal antibodies in the peroxidase anti-peroxidase technique on formalin-fixed LSG's from patients with SS and normal controls. Characteristic periductal staining for laminin occurred in the LSG's of 14/18 SS patients scored by one observer and 16/18 scored by the second observer. Staining of LSG's for laminin occurred in 2/35 control specimens consisting of 15 normal LSG's and 20 inflammatory lesions with attached normal LSG. The staining which occurred in the two controls was diffuse and 'non-specific' in one case, and indistinguishable from the characteristic periductal staining found in SS in the other case. Among the 20 controls containing inflammatory lesions, four showed diffuse staining for laminin within the actual lesion, but the adjacent LSG's did not stain. No statistically significant difference between SS and normal tissues stained by anti-fibronectin and anti-tubulin was observed. The study concluded that there was an increase in laminin or a laminin-like substance on salivary ductal epithelia of SS patients. This suggests a potential role for laminin in the pathologic mechanism and may indicate that increased laminin expression is a marker for SS.