Polymerase chain reaction amplification of bacterial 16s rRNA genes in prostate biopsies from men without chronic prostatitis
Polymerase chain reaction amplification of bacterial 16s rRNA genes in prostate biopsies from men without chronic prostatitis
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DOI：
10.1016/s0090-4295(98)00553-6
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发表时间：
1999-03-01
期刊：
影响因子：
2.1
通讯作者：
Alexander, RB
中科院分区：
文献类型：
--
作者：
Keay, S;Zhang, CO;Alexander, RB
目标。先前报道的一项使用巢式聚合酶链反应（PCR）分析的研究表明，在77%的慢性非细菌性前列腺炎患者的经会阴前列腺活检中存在多种原核微生物的DNA。由于该研究没有包括对照组，我们调查了从没有前列腺炎病史的男性中获得的经会阴前列腺活检是否也能发现微生物DNA。本文对9例局限性前列腺腺癌患者在超声引导下行前列腺双叶经会阴活检。从前列腺组织中提取DNA，并使用巢式引物从细菌16s rRNA基因的高度保守区域进行两轮扩增。对扩增的DNA进行纯化和测序，并将获得的序列与genbank中记录的细菌rRNA基因进行比较。9例患者中8例18例活检标本中11例PCR检测细菌DNA阳性。序列数据显示，11个标本中有8个标本为优势生物，与包括埃希氏菌和拟杆菌在内的几个不同属的细菌DNA同源性大于95%。活检前9例对照标本均为阴性。前列腺组织中细菌16s rRNA基因的存在并不是慢性前列腺炎所特有的，而是发生在大多数局限性前列腺癌患者中。这些细菌的存在是否与前列腺炎或前列腺癌等前列腺疾病的发展有关，需要仔细的对照试验，包括适当的对照组进行相同的检查。(C) 1999, Elsevier Science Inc.；版权所有。
Objectives. A previously reported study using nested polymerase chain reaction (PCR) analysis indicated the presence of DNA from a variety of prokaryotic microorganisms in 77% of transperineal prostate biopsies from patients with chronic nonbacterial prostatitis. Because that study did not include a control group, we investigated whether microbial DNA could also be found in transperineal prostate biopsies obtained from men who did not have a history of prostatitis.Methods. Transperineal biopsies of both lobes of the prostate were obtained under ultrasound guidance from 9 patients with localized adenocarcinoma of the prostate. DNA was extracted from the prostatic tissue and two-round amplification performed using nested primers from a highly conserved region of the bacterial 16s rRNA gene. Amplified DNA was purified and sequenced, and sequences obtained were compared to bacterial rRNA genes recorded in GenBank.Results. Eleven of 18 biopsy specimens from 8 of 9 patients were positive for bacterial DNA by PCR. Sequence data indicated a predominant organism in 8 of 11 specimens, with greater than 95% homology to DNA from several different genera of bacteria, including Escherichia and Bacteroides. All 9 control samples from the instruments before biopsy were negative.Conclusions. The presence of bacterial 16s rRNA genes in prostatic tissue is not specific for chronic prostatitis and occurred in most of our patients with localized prostate cancer. Whether the presence of such bacteria is related to the development of prostatic diseases such as prostatitis or prostatic cancer will require carefully controlled trials, including appropriate control groups examined identically. (C) 1999, Elsevier Science Inc. All rights reserved.