Comparative proteome profile of human placenta from normal and preeclamptic pregnancies.
Comparative proteome profile of human placenta from normal and preeclamptic pregnancies.
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DOI：
10.1371/journal.pone.0078025
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Liang G
中科院分区：
文献类型：
--
作者：
Wang F;Shi Z;Wang P;You W;Liang G
为了更好地了解先兆子痫胎盘病理发育的分子机制，我们使用LC-MS/MS构建了正常和先兆子痫妊娠人胎盘的大规模比较蛋白质组谱。在人类胎盘中共检测到2636种蛋白质，并且在对照和先兆子痫胎盘之间明确鉴定了171种不同的蛋白质。进一步的生物信息学分析表明，这些差异表达的蛋白质与子痫前期胎盘病理变化过程中的几个特异性细胞过程相关。随机选择6个蛋白质，用Western blotting验证其表达模式。其中3个蛋白质的细胞定位用免疫组织化学方法进行了验证。阐明蛋白质表达变化如何协调病理发展将为研究人员提供更好地了解先兆子痫的关键生物学过程和治疗药物调节胎盘功能的潜在靶点，并最终有利于先兆子痫的治疗。
To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placentas, and 171 different proteins were definitively identified between control and preeclamptic placentas. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes which occur during pathological changes of preeclamptic placenta. 6 proteins were randomly selected to verify their expression patterns with Western blotting. Of which, 3 proteins’ cellular localizations were validated with immunohistochemistry. Elucidation of how protein-expression changes coordinate the pathological development would provide researchers with a better understanding of the critical biological processes of preeclampsia and potential targets for therapeutic agents to regulate placenta function, and eventually benefit the treatment of preeclampsia.
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