Immunization with a DNA plasmid encoding the SAG1 (P30) protein of Toxoplasma gondii is immunogenic and protective in rodents
Immunization with a DNA plasmid encoding the SAG1 (P30) protein of Toxoplasma gondii is immunogenic and protective in rodents
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DOI：
10.1086/315186
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发表时间：
2000-01-01
影响因子：
6.4
通讯作者：
Kovacs, JA
中科院分区：
文献类型：
--
作者：
Angus, CW;Klivington-Evans, D;Kovacs, JA
DNA免疫可诱导体液免疫和细胞免疫应答，而体液免疫和细胞免疫在弓形虫免疫中起重要作用。为评价弓形虫表面抗原基因疫苗的免疫效果。免疫小鼠血清免疫荧光显示出对弓形虫速殖子的识别，ELISA检测到高滴度的SAG1抗体，SAG1刺激的小鼠脾细胞主要产生干扰素-γ和白介素2，免疫小鼠经80个ME49株组织包囊攻击后存活，而对照组小鼠全部死亡；20个组织包囊攻击导致脑囊变少，与对照组相比，素株卵囊免疫大鼠脑囊减少。强毒株速殖子对小鼠无保护作用，提示核酸疫苗对弓形虫感染有一定的保护作用。
Immunization with DNA can induce humoral and cell-mediated immune responses, both of which are important in conferring immunity to Taxoplasma gondii, The efficacy of genetic vaccination with a cDNA encoding the T. gondii SAG1 (P30) surface antigen was evaluated. Sera of immunized mice showed recognition of T. gondii tachyzoites by immunofluorescence and exhibited high titers of antibody to SAG1 by ELISA, SAG1-stimulated splenocytes from vaccinated mice produced primarily interferon-gamma and interleukin-2, Vaccinated mice survived challenge with 80 tissue cysts of ME49 strain, whereas all control mice died; challenge with 20 tissue cysts resulted in fewer brain cysts, compared with controls, Challenge of vaccinated rats with VEG strain oocysts resulted in a reduction in brain cysts. No protection was observed when mice were challenged with the highly virulent RH strain tachyzoites, These results suggest that nucleic acid vaccination can provide protection against T. gondii infection in mice.