Ion-beam and gamma-ray irradiations induce thermotolerant mutants in the entomopathogenic fungus Metarhizium anisopliae s.l.
Ion-beam and gamma-ray irradiations induce thermotolerant mutants in the entomopathogenic fungus Metarhizium anisopliae s.l.
复制标题
DOI：
10.1080/09583157.2014.918585
复制
发表时间：
2014-04
影响因子：
1.4
通讯作者：
Y. Fitriana;K. Satoh;I. Narumi;Tsutomu Saito
中科院分区：
文献类型：
--
作者：
Y. Fitriana;K. Satoh;I. Narumi;Tsutomu Saito
昆虫病原真菌，如金龟子绿僵菌（Metch.）索罗金是重要的害虫生物防治剂。然而，这些真菌与高温不相容。在这项研究中，使用离子束或γ射线诱变产生五个潜在的耐热突变体从两个野生型M。绿僵菌（两种使用离子束，三种使用伽马射线）。与野生型相比，突变体菌株具有更高的营养生长温度上限（2-3°C），并且增强了分生孢子萌发对45°C湿热胁迫的耐受性。在25°C和30°C下，大多数突变体对玉米象甲成虫的毒性与野生型一样，然而，使用离子束产生的一种突变体几乎完全失去毒性。这些结果表明，离子束和γ射线是有用的工具，用于改善生物学特性，如耐热性，在昆虫病原真菌，但突变体必须仔细评估不可预测的负面影响。
Entomopathogenic fungi, such as Metarhizium anisopliae (Metch.) Sorokin, are important agents for the biological control of insect pests. However, these fungi are not compatible with high temperatures. In this study, mutagenesis using ion beams or gamma rays was used to generate five potentially thermotolerant mutants from two wild-type isolates of M. anisopliae (two using ion beams and three using gamma rays). The mutant isolates had a higher upper thermal limit for vegetative growth compared to the wild types (by 2–3°C) and enhanced tolerance to wet–heat stress of 45°C for conidial germination. At 25°C and 30°C, most mutants were as virulent to maize weevil adults as the wild type, however, one mutant produced using ion beams almost lost virulence entirely. These results indicate that ion beams and gamma rays are useful tools for improving biological characteristics, such as thermotolerance, in entomopathogenic fungi, but that mutants must be carefully evaluated for unpredictable negative side effects.