An interferometric reflectance imaging sensor for point of care viral diagnostics.
An interferometric reflectance imaging sensor for point of care viral diagnostics.
复制标题
DOI：
10.1109/tbme.2013.2272666
复制
发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Ünlu MS
中科院分区：
文献类型：
--
作者：
Reddington AP;Trueb JT;Freedman DS;Tuysuzoglu A;Daaboul GG;Lopez CA;Karl WC;Connor JH;Fawcett H;Ünlu MS
体外诊断设备的使用正在从实验室过渡到初级保健环境，以满足早期疾病检测需求。由于当今标准检测所需的实验时间，时间关键的病毒诊断通常是在没有支持的情况下进行的。现有的快速护理点(POC)病毒检测的可靠性较低，需要后续的确证测试才能得出结论。为初级保健环境开发可靠的POC病毒检测将减少诊断时间，从而降低传播机会并改善康复。单粒子干涉反射成像传感器(SP-IRIS)是一种灵敏、特异的血清和全血检测平台。本文介绍了通过SP-IRIS原型向POC病毒检测迈出的一步，该原型具有自动数据采集和分析以及简单、易于使用的软件界面。操作复杂性的降低凸显了SP-IRIS作为一种灵敏而特异的POC诊断工具的潜力。由于集成了微流控芯片，这种自动化仪器将允许未经培训的用户在POC设置下运行样本到答案病毒检测。
The use of in vitro diagnostic devices is transitioning from the laboratory to the primary care setting to address early disease detection needs. Time critical viral diagnoses are often made without support due to the experimental time required in today’s standard tests. Available rapid point of care (POC) viral tests are less reliable, requiring a follow-on confirmatory test before conclusions can be drawn. The development of a reliable POC viral test for the primary care setting would decrease the time for diagnosis leading to a lower chance of transmission and improve recovery. The single particle interferometric reflectance imaging sensor (SP-IRIS) has been shown to be a sensitive and specific-detection platform in serum and whole blood. This paper presents a step towards a POC viral assay through a SP-IRIS prototype with automated data acquisition and analysis and a simple, easy-to-use software interface. Decreasing operation complexity highlights the potential of SP-IRIS as a sensitive and specific POC diagnostic tool. With the integration of a microfluidic cartridge, this automated instrument will allow an untrained user to run a sample-to-answer viral assay in the POC setting.