Epithelial Cell, Keratocyte, and Endothelial Cell Apoptosis in Fuchs' Dystrophy and in Pseudophakic Bullous Keratopathy
Epithelial Cell, Keratocyte, and Endothelial Cell Apoptosis in Fuchs' Dystrophy and in Pseudophakic Bullous Keratopathy
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福克斯营养不良和假性晶状体眼大疱性角膜病中的上皮细胞、角膜细胞和内皮细胞凋亡
DOI：
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发表时间：
2005
影响因子：
1.7
通讯作者：
Ildikó Süveges
中科院分区：
文献类型：
--
作者：
Nóra Szentmáry;B. Szende;B. Szende;Ildikó Süveges
目的通过检测Fuchs营养不良和人工晶状体大泡性角膜病变（pseudophakic bullous keratopathy，PBK）角膜各层细胞凋亡，探讨其病理机制。方法对21例（21眼）中央穿透性角膜移植术后获得的角膜瓣进行研究，其中Fuchs营养不良13例（14眼，年龄70.8 ± 10.0岁），PBK 7例（7眼，年龄69.6 ± 10.2岁）。来自患有脉络膜黑色素瘤的摘除眼的四个纽扣作为对照。HE染色观察组织学变化。使用TUNEL（末端脱氧核糖核苷酸转移酶介导的dUTP-地高辛缺口末端标记）测定法测定上皮层、基质层和内皮层单独样本中每个视野（125倍放大率）的凋亡细胞平均数。结果在11例Fuchs'营养不良角膜和2例PBK角膜中检测到凋亡活性。与对照相比，Fuchs营养不良角膜中所有三层的平均（标准化）凋亡细胞数（每种情况下p=0.01）和PBK角膜中基质层的平均（标准化）凋亡细胞数（p<0.01）在统计学上有显著差异。后者的上皮层和内皮层的凋亡细胞数较高，但差异无统计学意义（p=0.07，0.07）。结论细胞凋亡可能参与了角膜营养不良的发病机制，并参与了角膜基质细胞的死亡。
Purpose To elucidate the pathomechanism of Fuchs' dystrophy and pseudophakic bullous keratopathy (PBK) by examining cell apoptosis in different corneal layers. Methods The authors studied corneal buttons obtained from 21 eyes following central penetrating keratoplasty: 14 corneal buttons (13 patients, age 70.8 ± 10.0 years) with Fuchs' dystrophy, and 7 buttons (7 patients, age 69.6 ± 10.2 years) with PBK. Four buttons from enucleated eyes with choroidal melanoma served as controls. Histologic changes were examined using light microscopy with hematoxylin-eosin (HE) staining. The average numbers of apoptotic cells per field of view (125x magnification) in separate samples of the epithelial, stromal, and endothelial layers were determined using the TUNEL (terminal deoxyri-bonucleotidyl transferase-mediated dUTP-digoxigenin nick-end labeling) assay. Results In 11 of the Fuchs' dystrophy corneas and 2 of the PBK corneas, apoptotic activity was detected. In the control corneas no apoptotic activity was found. Compared to the controls there was a statistically significant difference in the mean (normalized) apoptotic cell numbers for all three layers (p=0.01 in each case) in the Fuchs' dystrophy corneas, and for the stromal layer (p<0.01) in PBK corneas. The apoptotic cell numbers for the epithelial and endothelial layers of the latter were higher, but the difference was not statistically significant (p=0.07, 0.07). Conclusions Apoptosis may play a role in the pathomechanism of Fuchs' dystrophy and in keratocyte death in corneas with PBK.
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影响因子：
4.4
作者：
S. Wilson;W. J. Kim
通讯作者：
S. Wilson;W. J. Kim
细胞凋亡在 Fuchs 角膜内皮营养不良发病机制中的作用。
影响因子：
--
作者：
Li,QJ;Ashraf,MF;Shen,DF;Green,WR;Stark,WJ;Chan,CC;O'Brien,TP
通讯作者：
O'Brien,TP
影响因子：
3.5
作者：
Biswas, S;Munier, FL;Black, GCM
通讯作者：
Black, GCM
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影响因子：
4.4
作者：
Rosenbaum,JT;Planck,ST;Huang,XN;Rich,L;Ansel,JC
通讯作者：
Ansel,JC
福克斯营养不良中的房水成分。
影响因子：
4.4
作者：
Wilson,SE;Bourne,WM;Maguire,LJ;Rahhal,FM;Ribaudo,RK;Kreutzer,DL;O'Rourke,J
通讯作者：
O'Rourke,J