Distinguishing between archaeological sheep and goat bones using a single collagen peptide
Distinguishing between archaeological sheep and goat bones using a single collagen peptide
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DOI：
10.1016/j.jas.2009.08.020
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Collins, Matthew
中科院分区：
文献类型：
--
作者：
Buckley, Mike;Kansa, Sarah Whitcher;Collins, Matthew
我们描述了一种分离和分析能够区分绵羊和山羊骨胶原的单一胶原肽的方法。对来自绵羊和山羊胶原蛋白的33个氨基酸的肽进行测序，并显示两个物种在两个位置上不同。一系列的山羊的分析表明，序列变化之间的分歧发生的喜马拉雅塔尔（Hemitragus jemlahicus）和北山羊（山羊），并建议山羊标记是诊断所有山羊物种和品种。这些标志物在考古骨骼中的生存进行了测试，使用一组26 ovicaprid标本从Domuztepe，在土耳其中南部的新石器时代遗址。这些标记物用于测试24个Domuztepe骨骼的骨骼学测定，并确定两个未成熟标本的物种。由于胶原蛋白比其他生物分子（如古DNA）长期存活，因此胶原蛋白-肽方法比其他非形态学方法具有优势。研究结果还强调了依靠一个形态学标准来区分绵羊骨和山羊骨的问题，在这种情况下，这一标准是桡骨远端。(C)2009爱思唯尔有限公司保留所有权利。
We describe a method of isolating and analyzing a single collagen peptide able to distinguish between sheep and goat bone collagen. The 33 amino acid peptide from both sheep and goat collagen was sequenced and shown to differ between the two species at two positions. Analysis of a range of caprines indicated that the sequence changes occurred between the divergence of the Himalayan tahr (Hemitragus jemlahicus) and the ibex (Capra ibex) and that the proposed goat marker is diagnostic of all Capra species and breeds. The survival of these markers in archaeological bones was tested using a set of 26 ovicaprid specimens from Domuztepe, a Neolithic site in south central Turkey. These markers were used to test the osteological determination of 24 of the Domuztepe bones, and determine the species for two immature specimens. The collagen-peptide method has advantages over other non-morphological methods of sheep/goat distinction because of the long-term survival of collagen over other biomolecules such as ancient DNA. The results also highlighted the problems in relying upon one morphological criterion, in this case on the distal radius, to distinguish between sheep and goat bones. (C) 2009 Elsevier Ltd. All rights reserved.