Effects of PEGylated porcine glucagon-like peptide-2 therapy in weaning piglets challenged with lipopolysaccharide
Effects of PEGylated porcine glucagon-like peptide-2 therapy in weaning piglets challenged with lipopolysaccharide
复制标题
聚乙二醇化猪胰高血糖素样肽 2 治疗对脂多糖攻击的断奶仔猪的影响
DOI：
10.1016/j.peptides.2014.05.007
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发表时间：
2014-08-01
期刊：
影响因子：
3
通讯作者：
Xu, Zi-wei
中科院分区：
文献类型：
--
作者：
Qi, Ke-ke;Wu, Jie;Xu, Zi-wei
This study aims to evaluate the therapeutic effect of polyethylene glycosylated porcine glucagon-like peptide-2 (pGLP-2), a long-acting form of pGLP-2, in lipopolysaccharide (LPS)-challenged piglets. Eighteen 21-day-old weaning piglets were randomly assigned into three groups: control (saline solution), LPS (100 mu g/kg LPS), and PEG-pGLP-2 (10 nmol/kg PEG-pGLP-2 + 100 mu g/kg LPS). All treatments were administered intraperitoneally. Compared with the control treatment, LPS treatment significantly decreased (P < 0.05) the villus heights of the duodenum and jejunum, as well as the villus height/crypt depth ratio of the jejunum. However, PEG-pGLP-2 therapy reduced these effects (P > 0.05). Specifically, PEG-pGLP-2 infusion significantly increased the villus height/crypt depth ratio of the duodenum (P < 0.05) compared with LPS treatment. Compared with the control treatment, LPS treatment significantly increased (P < 0.05) the mRNA expression levels of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) in the jejunum. However, PEG-pGLP-2 therapy reduced these effects (P < 0.05). Specifically, PEG-pGLP-2 infusion significantly decreased (P < 0.05) the mRNA expression levels of interleukin (IL)-8 and TNF-alpha in the duodenum and jejunum, IL-10 in the duodenum, and IFN-gamma in the jejunum compared with the LPS treatment. LPS treatment increased the caspase-3 activity of the ileum mucosal (P < 0.05), and this effect was significantly reduced by PEG-pGLP-2 treatment. These results indicate that PEG-pGLP-2 infusion alleviates the severity of intestinal injury in weaning piglets by reducing the secretion of inflammatory cytokines and the caspase-3 activity, and increasing the villus height/crypt depth ratio. (C) 2014 Elsevier Inc. All rights reserved.
