The potential of trehalose to replace insect hemolymph in artificial media for Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae)
The potential of trehalose to replace insect hemolymph in artificial media for Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae)
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DOI：
10.1111/j.1744-7917.2012.01566.x
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发表时间：
2013-10-01
期刊：
影响因子：
4
通讯作者：
De Clercq, Patrick
中科院分区：
文献类型：
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作者：
Lu, Xin;Han, Shi-Chou;De Clercq, Patrick
昆虫添加剂已被证明可以提高赤眼蜂物种人工培养基的价值，但同时保持对宿主昆虫平行培养的依赖。在含有不同含量柞蚕蛹血淋巴的人工培养基上，添加蒸馏水、海藻糖水溶液和格雷斯昆虫培养基，对松毛虫赤眼蜂（Trichogramma dendrolimi Matsumura）从卵到成虫进行离体饲养。结果表明，昆虫血淋巴是人工培养基的关键成分。发育参数，包括寄生率，化蛹，成人出现和正常成人，和生产的成年人的数量，增加补充海藻糖的培养基上，即使蛹血淋巴的比例降低。两种人工培养基，第一种含30%血淋巴和10%海藻糖的水溶液，寄生率98.9%，化蛹率77.7.0%，羽化率77.2%，正常成虫率80.0%，成虫333头;第二种含25%血淋巴和15%海藻糖的Grace昆虫培养基，寄生率97.8%。化蛹率91.0%，羽化率85.2%，正常成蜂率76.1%，生产成虫757头，具有大规模生产的潜力。松毛虫使用海藻糖部分取代蛹血淋巴在人工培养基中的这种和其他赤眼蜂物种可能有助于显着降低其生产成本，并可能因此有助于避免与鳞翅目卵供应短缺有关的问题，目前构成了大量饲养寄生蜂的主要生产宿主。
Insect additives have been shown to improve the value of artificial media for Trichogramma species, but at the same time maintain dependence on parallel cultures of host insects. In the present study, Trichogramma dendrolimi Matsumura was reared in vitro from egg to adult on artificial media with different contents of pupal hemolymph of Chinese oak silkmoth Antheraea pernyi (Guerin-Meneville) and with supplements of distilled water, or of trehalose dissolved in water or in Grace's insect medium. The results indicated insect hemolymph was the key component of artificial medium. Developmental parameters, including rates of parasitism, pupation, adult emergence and normal adults, and numbers of produced adults, were increased on media supplemented with trehalose even when the proportion of pupal hemolymph was reduced. Two artificial media, the first containing 30% hemolymph and 10% trehalose in water with 98.9% parasitism rate, 77.7.0% pupation rate, 77.2% emergence rate, 80.0% normal adult rate and 333 produced adults, and the second containing 25% hemolymph and 15% trehalose in Grace's insect medium with 97.8% parasitism rate, 91.0% pupation rate, 85.2% emergence rate, 76.1% normal adult rate and 757 produced adults, were believed to hold potential to mass produce T. dendrolimi. The use of trehalose to partially replace pupal hemolymph in artificial medium of this and other Trichogramma species may contribute to a significant reduction in their production cost and may as such help to evade problems related to short supplies of lepidopteran eggs, which currently constitute the main factitious host for the mass rearing of the parasitoids.