A Mutant Plasma Membrane Protein Is Stabilized Upon Loss of Yvh1, a Novel Ribosome Assembly Factor
A Mutant Plasma Membrane Protein Is Stabilized Upon Loss of Yvh1, a Novel Ribosome Assembly Factor
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DOI：
10.1534/genetics.108.100099
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发表时间：
2009-03-01
期刊：
影响因子：
3.3
通讯作者：
Chang, Ainy
中科院分区：
文献类型：
--
作者：
Liu, Yu;Chang, Ainy
Pma1-10是一种突变的质膜atp酶，在细胞表面的稳定性受到限制温度的影响。在37度时，Pma1-10被泛素化并从质膜内化，在液泡中降解。编码酪氨酸磷酸酶的YVH1是pma1-10的突变抑制因子；在缺乏Yvh1的情况下，Pma1-10在质膜上保持稳定，使细胞得以生长。将突变体Pma1-10从质膜上移除需要Yvh1的RING finger结构域，而不是其磷酸酶结构域。Yvh1是一种新型核糖体组装因子；yvh1 δ细胞，游离60S和80S核糖体亚基减少，游离40S亚基增加，半聚体增多。Pma1-10也通过缺失60S核糖体蛋白Rp119a和Rp135a来稳定。我们提出，由于Yvh1或特定核糖体蛋白的丢失而引起的核糖体生物发生的变化可能通过产生特定的翻译变化而对质膜产生影响。
Pma1-10 is a mutant plasma membrane ATPase defective at the restrictive temperature in stability at the cell surface. At 37 degrees, Pma1-10 is ubiquitinated and internalized from the plasma membrane for degradation in the vacuole. YVH1, encoding a tyrosine phosphatase, is a mutant suppressor of pma1-10; in the absence of Yvh1, Pma1-10 remains stable at the plasma membrane, therapy permitting cells to grow. The RING finger domain of Yvh1, but not its phosphatase domain, is required for removal of mutant Pma1-10 from the plasma membrane. Yvh1 is a novel ribosome assembly factor; and yvh1 Delta cells, free 60S and 80S ribosomal subunits are decreased free 40S subunits are increased, and half-mer polysomes are accumulated. Pma1-10 is also stabilized by deletion of 60S ribosomal proteins Rp119a and Rp135a. We propose that changes in ribosome biogenesis caused by loss Yvh1 or specific ribosomal proteins have effects on the plasma membrane, perhaps by producing specific translational changes.