Dexamethasone downregulates vitamin D receptors in rat kidney, unmasking a high affinity binding site.
Dexamethasone downregulates vitamin D receptors in rat kidney, unmasking a high affinity binding site.
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地塞米松下调大鼠肾脏中的维生素 D 受体，揭示高亲和力结合位点。
DOI：
10.1006/bbrc.1993.2163
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发表时间：
1993
影响因子：
3.1
通讯作者：
Walters,MR
中科院分区：
文献类型：
--
作者：
Gensure,RC;Fish,JM;Walters,MR
地塞米松对大鼠维生素D受体水平产生组织特异性影响：肠中的受体上调，肾脏中的受体下调，而对睾丸、心脏和肺中的受体无影响。Scatchard分析显示，肾脏中低亲和力受体位点（Kd=0.9 nM）的选择性下调，从而揭示了高亲和力结合位点（Kd=0.05 nM）。肠中的单个低亲和力位点（Kd=0.3-0.7 nM）上调，而睾丸中的单个高亲和力位点（Kd = 0.07-0.09 nM）没有改变。这些结果表明，在大鼠肾脏中存在两种类型的核结合位点，并建立在大鼠肾脏中的高亲和力结合位点和睾丸中的单个高亲和力受体位点之间存在相似性。
Dexamethasone exerted tissue-specific effects on rat vitamin D receptor levels: upregulation of receptors in intestine, downregulation in kidney, and no effect on receptors in testis, heart, and lung. Scatchard analysis showed selective downregulation of the low affinity receptor site (Kd=0.9 nM) in kidney, thus unmasking a high affinity binding site (Kd=0.05 nM). The single low affinity site in intestine (Kd=0.3-0.7 nM) was upregulated, while the single high affinity site in testis (Kd = 0.07-0.09 nM) was not changed. These results demonstrate the existence of two types of nuclear binding sites in rat kidney and establish that there are similarities between the high affinity binding site in the rat kidney and the single high affinity receptor site in the testis.