Inactivation of Norovirus by ozone gas in conditions relevant to healthcare
Inactivation of Norovirus by ozone gas in conditions relevant to healthcare
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DOI：
10.1016/j.jhin.2006.12.021
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发表时间：
2007-05-01
影响因子：
6.9
通讯作者：
Petric, M.
中科院分区：
文献类型：
--
作者：
Hudson, J. B.;Sharma, M.;Petric, M.
我们评估了臭氧气体对放置在酒店房间、游轮客舱和办公室内不同位置的干燥样品中的诺如病毒及其动物替代物猫杯状病毒（FCV）的杀灭能力。诺如病毒通过定量逆转录酶实时聚合酶链反应（QRT-PCR）测定，FCV通过QRT-PCR和病毒感染性测定的组合测定。在臭氧暴露的最佳条件下，我们能够将传染性FCV的浓度降低103倍以上，在某些情况下甚至无法检测到，总操作时间不到一个小时。QRT-PCR测定表明两种病毒RNA的降低相似。在硬表面（塑料、钢和玻璃）和软表面（如织物、棉花和地毯）上干燥的含病毒样品同样容易受到处理。我们的研究结果表明，诺如病毒可以通过暴露于便携式商业发生器中的臭氧气体而灭活，例如酒店房间，游轮客舱和医疗保健设施。(C)2007年，医院感染协会。出版社：Elsevier Ltd.
We evaluated the ability of ozone gas to inactivate Norovirus and its animal surrogate feline calicivirus (FCV) in dried samples placed at various locations within a hotel room, a cruise liner cabin and an office. Norovirus was measured by quantitative reverse transcriptase real-time polymerase chain reaction (QRT-PCR) assay, and FCV by a combination of QRT-PCR and virus infectivity assays. We were able to reduce the concentration of infectious FCV by a factor of more than 103, and in some cases beyond detection, under optimal conditions of ozone exposure with less than an hour of total operation. QRT-PCR assays indicated similar decreases in both viral RNAs. Virus-containing samples dried onto hard surfaces (plastic, steel and glass), and soft surfaces such as fabric, cotton and carpet, were equally vulnerable to the treatment. Our results show that Norovirus can be inactivated by exposure to ozone gas from a portable commercial generator in settings such as hotel rooms, cruise ship cabins and healthcare facilities. (C) 2007 The Hospital Infection Society. Published by Elsevier Ltd. All rights