Increasing the Lifetime of Insulin Cannula with Antifouling and Nitric Oxide Releasing Properties
Increasing the Lifetime of Insulin Cannula with Antifouling and Nitric Oxide Releasing Properties
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DOI：
10.1021/acsabm.9b00908
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发表时间：
2019-12-16
影响因子：
4.7
通讯作者：
Brisbois, Elizabeth J.
中科院分区：
文献类型：
--
作者：
Chug, Manjyot Kaur;Feit, Corbin;Brisbois, Elizabeth J.
许多1型糖尿病患者使用胰岛素泵，其依赖于小的皮下胰岛素输注套管。然而，胰岛素套管仍然遭受感染和炎症，这影响胰岛素套管的佩戴时间，降低胰岛素输注的效率，并且需要频繁旋转胰岛素输注部位。持续胰岛素输注泵治疗的感染和炎症是日益严重的问题，估计每年全球花费数十亿美元。本研究旨在开发一种具有生物启发聚合物协同效应的强效抗菌和抗微生物胰岛素套管，将光滑的液体注入多孔表面技术与活性一氧化氮（NO）释放聚合物相结合。通过溶剂浸渍工艺将NO供体分子S-亚硝基-N-乙酰青霉胺（SNAP）和硅油（Si）浸渍在商业医用级硅橡胶（SR）管（SR-SNAP-Si）中，开发了套管。使用体外生物测定研究了SR-SNAP-Si减少蛋白质吸附并提供针对金黄色葡萄球菌和表皮葡萄球菌的抗菌特性的效率。SR-SNAP-Si套管在生理水平下释放NO超过14 d，在室温下储存30 d稳定。扫描电子显微镜图像显示在溶剂浸渍过程后材料表面没有可观察到的变化。硅油的注入使蛋白质在套管上的吸附量降低了66.40%，NO的释放降低了S. epidermidis和S.与SR对照相比，24 h后的金黄色葡萄球菌的细胞增殖率分别为94.89%和99.77%。这种胰岛素套管提供了连续的NO释放和超过14 d的粘附界面，并表现出显着减少蛋白质和细菌粘附。这种开发用于皮下胰岛素输注套管的双功能一氧化氮释放和表面活性剂的方法具有减少与胰岛素泵输送系统相关的感染和炎症的巨大潜力。
Many Type 1 diabetes patients utilize insulin pumps, which rely on a small subcutaneous insulin infusion cannula. However, insulin cannulas still suffer from infection and inflammation, which impacts the wear time of the insulin cannula, reduces the efficiency of insulin infusion, and requires frequent rotation of the insulin infusion site. Infection and inflammation of continuous insulin infusion pump therapy are growing issues and are estimated to cost billions of dollars globally each year. This study aims to develop a potent antibacterial and antifouling insulin cannula with a synergistic effect of bioinspired polymers, integrating antifouling slippery, liquid-infused porous surface technology with an active nitric oxide (NO) releasing polymer. The cannulas were developed by impregnating the NO donor molecule S-nitroso-N-acetylpenicillamine (SNAP) and silicone oil (Si) in commercial medical-grade silicone rubber (SR) tubing (SR-SNAP-Si) via a solvent-impregnation process. The efficiency of the SR-SNAP-Si to reduce protein adsorption and provide antibacterial properties against Staphylococcus aureus and Staphylococcus epidermidis were studied using in vitro bioassays. The SR-SNAP-Si cannula released NO for more than 14 d at physiological levels and were stable during storage for 30 d at room temperature. Scanning electron microscopy images revealed no observable changes to the material surface after the solvent impregnation process. The infusion of silicone oil significantly reduced the protein adsorption on the cannula by 66.40%, and the NO release reduced the viable bacterial cell adhesion of S. epidermidis and S. aureus after 24 h by 94.89% and 99.77%, respectively, as compared to SR controls. This insulin cannula provided continuous NO release and an antifouling interface for more than 14 d and exhibited significant reduction in protein and bacterial adhesion. This method of developing dual-function nitric oxide releasing and antifouling surface for subcutaneous insulin infusion cannulas holds great potential to reduce infection and inflammation associated with insulin pump delivery systems.