DIETARY LIPIDS, PROSTAGLANDIN-E(2) LEVELS, AND TOOTH MOVEMENT IN ALVEOLAR BONE OF RATS
DIETARY LIPIDS, PROSTAGLANDIN-E(2) LEVELS, AND TOOTH MOVEMENT IN ALVEOLAR BONE OF RATS
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DOI：
10.1007/bf01351839
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发表时间：
1993-11-01
影响因子：
4.2
通讯作者：
ALAM, SQ
中科院分区：
文献类型：
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作者：
KOKKINOS, PP;SHAYE, R;ALAM, SQ
先前的一项研究表明，某些膳食脂质可以改变牙槽骨中的花生四烯酸浓度。由于花生四烯酸是前列腺素 (PG) E2 的前体，已知前列腺素 E2 在正畸牙齿移动中发挥重要作用，因此本研究的目的是确定膳食脂质对 PGE、水平和牙齿移动的影响。两组雄性 Sprague-Dawley 大鼠（20 只/组）喂食营养充足的纯化饮食，其中含有 10% 玉米油（第 I 组，富含 n-6 脂肪酸）或 9% n-3 脂肪酸乙酯浓缩物 +1% 玉米油（第 II 组，富含 n-3 脂肪酸）。饲喂该饮食 5 周后，对上颌切牙施加 56 g 的正畸力，使其向远中倾斜。在施加正畸力的第4天和第8天处死大鼠之前，通过计算机图像分析测量牙齿移动。解剖前上颌骨，除去软组织和门牙。将牙槽骨冷冻在液氮中，粉碎并提取脂质。通过气相色谱法测定总磷脂的花生四烯酸和脂肪酸组成的浓度。 PGE2水平通过酶免疫测定法测量。 II组大鼠牙槽骨中花生四烯酸和PGE浓度显着低于I组（P < 0.001）。第4天和8天时，II组大鼠的牙齿移动也显着低于I组（P < 0.02）。结果表明，牙槽骨和正畸牙齿移动中的 PGE2 水平可能受到膳食脂肪类型的影响。
A previous study showed that certain dietary lipids can alter arachidonic acid concentrations in alveolar bone. Because arachidonic acid is a precursor of prostaglandin (PG) E2, which is known to play an important role in orthodontic tooth movement, the purpose of the present study was to determine the effect of dietary lipids on PGE, levels and tooth movement. Two groups of male Sprague-Dawley rats (20/group) were fed nutritionally adequate purified diets containing 10% corn oil (group I, rich in n-6 fatty acids) or 9% ethyl ester concentrate of n-3 fatty acids +1% com oil (group II rich in n-3 fatty acids). After 5 weeks of feeding the diets, orthodontic force of 56 g was applied to the maxillary incisors to tip them distally. Prior to killing the rats at day 4 and 8 of orthodontic force application, tooth movement was measured by computerized image analysis. Pre-maxillae were dissected out free of soft tissue and incisors. The alveolar bone was frozen in liquid nitrogen, pulverized, and lipids were extracted. The concentrations of arachidonic acid and fatty acid composition of total phospholipids were measured by gas chromatography. PGE2 levels were measured by enzyme immunoassay. Arachidonic acid and PGE, concentration were significantly lower (P < 0.001) in alveolar bone of rats in group II than in group I. The tooth movement was also significantly lower (P < 0.02) in group II than in group I at both 4 and 8 days. The results suggest that PGE2 levels in alveolar bone and orthodontic tooth movement can be affected by the type of dietary fat.