Analysis of genetic diversity among some Persian walnut genotypes (Juglans regia L.) using morphological traits and SSRs markers
Analysis of genetic diversity among some Persian walnut genotypes (Juglans regia L.) using morphological traits and SSRs markers
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DOI：
10.1016/j.scienta.2011.06.028
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发表时间：
2011-08-26
影响因子：
4.3
通讯作者：
Zamani, Zabihollah
中科院分区：
文献类型：
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作者：
Ebrahimi, Aziz;Fatahi, Reza;Zamani, Zabihollah
伊朗栽培核桃树的多样性很高，这是由于其种子繁殖时间长，栽培面积大。本研究利用形态学特征和SSR标记对31个伊朗核桃基因型沿着和4个国外品种的遗传多样性和亲缘关系进行分析。结果表明，不同基因型的核桃仁重、仁重和出仁率分别为7.52 ~ 17.73g、4.00 ~ 9.83g和38.78 ~ 67.05%。在SSR分析中，9对引物检测到39个等位基因，从2到8不等，平均值为5.10个等位基因每个引物。伊朗基因型在SSR位点和形态性状上都表现出较高的多样性。虽然外国品种（'Serr'，'Vina'，'Franquette'和'Lara'）彼此聚类，但它们也靠近伊朗基因型。研究结果为我们提供了宝贵的多样性，我们的基因型，可用于育种研究，也显示了遗传标记的力量，分析和评价这种多样性。(C)2011 Elsevier B. V.保留所有权利。
There is a high diversity among cultivated walnut trees in Iran due to its long time of seed propagation and vast area of cultivation. In this study some morphological characters as well as Simple Sequence Repeat (SSRs) markers were used to analyze the genetic diversity and relationships among 31 Iranian walnut genotypes along with four foreign cultivars. The nut weight ranged from 7.52 to 17.73 g, kernel weight from 4.00 to 9.83 g, and kernel percentage ratio from 38.78 to 67.05% among studied genotypes. In SSRs analysis, nine primer pairs were tested that produced 39 alleles ranging from 2 to 8, with a mean value of 5.10 allele per primer. The Iranian genotypes showed relatively high diversity both for their SSRs loci and morphological traits. Although the foreign cultivars ('Serr', 'Vina', 'Franquette' and 'Lara') clustered with each other, they also laid close and within the Iranian genotype. The results of the study provided us with valuable diversity among our genotypes which could be used for breeding studies and also showed the power of genetic markers for analysis and evaluation of this diversity. (C) 2011 Elsevier B.V. All rights reserved.