Effects of the F-actin inhibitor latrunculin A on the budding yeast Saccharomyces cerevisiae.
Effects of the F-actin inhibitor latrunculin A on the budding yeast Saccharomyces cerevisiae.
复制标题
F-肌动蛋白抑制剂 latrunculin A 对出芽酵母酿酒酵母的影响。
DOI：
10.1099/mic.0.000091
复制
发表时间：
2015
期刊：
影响因子：
1.5
通讯作者：
S. Kawamoto
中科院分区：
文献类型：
--
作者：
M. Kopecká;M. Yamaguchi;S. Kawamoto
我们的基础细胞生物学研究旨在研究F-肌动蛋白细胞骨架突然丢失对真核细胞的影响。应用相差显微镜、荧光显微镜、冷冻替代法、透射和扫描电子显微镜，在Bürker小室计数细胞数并测量吸光度，观察了在酵母提取液中加入冬凌草甲素(LA)的细胞。细胞对F-肌动蛋白抑制剂LA的存在有反应，肌动蛋白斑块、肌动蛋白缆线和肌动蛋白环消失。细胞壁形态不规则，细胞器和分泌泡超微结构紊乱。LA抑制的细胞只形成“桌山状”的扁平膨大，没有顶端生长，细胞壁薄，含有β-1，3-葡聚糖微纤维。LA抑制的细胞裂解。花蕾的形成和生长需要肌动蛋白缆线和贴片。此外，在芽细胞壁上形成β-1，3-葡聚糖微纤维需要肌动蛋白贴片。La对发芽酵母酿酒酵母具有抑菌、杀菌和杀菌作用。
Our basic cell biology research was aimed at investigating the effect on eukaryotic cells of the sudden loss of the F-actin cytoskeleton. Cells treated with latrunculin A (LA) in yeast extract peptone dextrose (YEPD) medium were examined using phase-contrast and fluorescent microscopy, freeze-substitution, transmission and scanning electron microscopy, counted using a Bürker chamber and their absorbance measured. The cells responded to the presence of LA, an F-actin inhibitor, with the disappearance of actin patches, actin cables and actin rings. This resulted in the formation of larger spherical cells with irregular morphology in the cell walls and ultrastructural disorder of the cell organelles and secretory vesicles. Instead of buds, LA-inhibited cells formed only 'table-mountain-like' wide flattened swellings without apical growth with a thinner glucan cell-wall layer containing β-1,3-glucan microfibrils. The LA-inhibited cells lysed. Actin cables and patches were required for bud formation and bud growth. In addition, actin patches were required for the formation of β-1,3-glucan microfibrils in the bud cell wall. LA has fungistatic, fungicidal and fungilytic effects on the budding yeast Saccharomyces cerevisiae.
酵母肌动蛋白基因温度敏感突变等位基因的构建和遗传特征。
DOI：
10.1073/pnas.81.15.4889
发表时间：
1984
影响因子：
11.1
作者：
Shortle,D;Novick,P;Botstein,D
通讯作者：
Botstein,D