Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks
Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks
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DOI：
10.1016/j.biortech.2012.05.112
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发表时间：
2012-09-01
影响因子：
11.4
通讯作者：
Martinez, Angel T.
中科院分区：
文献类型：
--
作者：
Gutierrez, Ana;Rencoret, Jorge;Martinez, Angel T.
以1-羟基苯并三氮唑（HBT）为介体，采用碱抽提法，研究了毛栓菌漆酶对木质素的去除作用。在50 U g（-1）漆酶和2.5%HBT浓度下，桉木和狼尾草木质素的去除率分别为48%和32%。在二甲基亚砜-d（6）中溶胀的原料的二维核磁共振显示，对羟基苯基、愈创木基和双辛基木质素单元和木质素的脂族（主要是β-O-4 '-连接的）侧链被去除，对香豆酸（存在于狼尾草中）被适度去除，而多糖交叉信号没有实质性变化。酶预处理（在25 U g-1）的桉树和狼尾草原料增加葡萄糖（61%和12%，在72小时）和乙醇（4和2 g L-1，在17小时）产量从两个木质纤维素材料，分别相比，那些没有酶处理。(C)2012爱思唯尔有限公司保留所有权利。
The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g(-1) laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d(6), revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly beta-O-4'-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g-1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L-1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment. (C) 2012 Elsevier Ltd. All rights reserved.