Hydrogen-rich water improves sleep consolidation and enhances forebrain neuronal activation in mice.
Hydrogen-rich water improves sleep consolidation and enhances forebrain neuronal activation in mice.
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DOI：
10.1093/sleepadvances/zpad057
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发表时间：
2024
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睡眠不足会导致各种健康问题，损害神经功能。最近，氢分子作为一种无毒的人体健康促进剂而广受欢迎。氢分子对睡眠和与睡眠相关的神经系统的影响仍未被探索。本研究探讨富氢水（HRW）对睡眠剥夺小鼠睡眠行为和神经元激活的影响。采用脑电图（EEG）和肌电（EMG）记录电极植入成年C57BL/6J小鼠，并给予HRW （0.7 ~ 1.4 mM）或常规水，连续7天，不受限制。在基线条件下和急性睡眠缺失后记录睡眠-觉醒周期。使用cFos免疫染色评估睡眠和清醒相关区域的神经元激活。HRW增加了未受干扰小鼠的睡眠巩固，增加了睡眠剥夺小鼠的非快速眼动和快速眼动睡眠量。HRW还减少了小鼠在光线照射后入睡的平均时间。所有小鼠经HRW处理后，外侧鼻中隔、内侧鼻中隔、腹外侧视前区和正中视前区的神经元激活均发生显著改变。HRW改善了睡眠巩固，增加了与睡眠相关的大脑区域的神经元激活。它可以作为一种简单有效的治疗方法来改善睡眠不足后的恢复。
Sleep loss contributes to various health issues and impairs neurological function. Molecular hydrogen has recently gained popularity as a nontoxic ergogenic and health promoter. The effect of molecular hydrogen on sleep and sleep-related neural systems remains unexplored. This study investigates the impact of hydrogen-rich water (HRW) on sleep behavior and neuronal activation in sleep-deprived mice. Adult C57BL/6J mice were implanted with electroencephalography (EEG) and electromyography (EMG) recording electrodes and given HRW (0.7–1.4 mM) or regular water for 7 days ad libitum. Sleep–wake cycles were recorded under baseline conditions and after acute sleep loss. Neuronal activation in sleep- and wake-related regions was assessed using cFos immunostaining. HRW increased sleep consolidation in undisturbed mice and increased non-rapid-eye movement and rapid-eye-movement sleep amount in sleep-deprived mice. HRW also decreased the average amount of time for mice to fall asleep after light onset. Neuronal activation in the lateral septum, medial septum, ventrolateral preoptic area, and median preoptic area was significantly altered in all mice treated with HRW. HRW improves sleep consolidation and increases neuronal activation in sleep-related brain regions. It may serve as a simple, effective treatment to improve recovery after sleep loss.