Selection of Streptomyces isolates from Turkish karstic caves against antibiotic resistant microorganisms.
Selection of Streptomyces isolates from Turkish karstic caves against antibiotic resistant microorganisms.
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从土耳其喀斯特洞穴中选择链霉菌分离株来对抗抗生素抗性微生物。
DOI：
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发表时间：
2010
影响因子：
0.8
通讯作者：
M. Yamaç
中科院分区：
文献类型：
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作者：
S. Yücel;M. Yamaç
在这项工作中，从土耳其 19 个喀斯特洞穴的岩壁和洞穴表面以及土壤样本中分离出了放线菌。在筛选项目中，分离出 290 个分离株，其中 180 个分离株 (62%) 对四种细菌、两种酵母菌和四种丝状真菌表现出抗菌活性。其中之一，链霉菌。 1492，检查了分批培养中抗生素的生产。第 5 天显示最大抗菌活性。提取的活性化合物的抗菌活性被记录为对抗生素耐药临床菌株的剂量依赖性抑菌或杀菌作用；耐甲氧西林金黄色葡萄球菌 (MRSA)、耐万古霉素屎肠杆菌 (VRE) 和鲍曼不动杆菌。最低抑制剂浓度和最低杀菌浓度测定为低于标准抗生素链霉素；分别为 125 微克/毫升和 250-1000 微克/毫升。发现活性成分具有热稳定性。
In this work, actinomycetes isolates were isolated from rock wall and speleothem surfaces and soil samples of 19 karstic caves in Turkey. Out of 290 isolates isolated, 180 isolates (62%) exhibited antimicrobial activity against a panel of four bacteria, two yeasts and four filamentous fungi in the screening program. One of them, Streptomyces sp. 1492, was examined for antibiotic production in batch culture. The maximum of antimicrobial activity was shown at 5th day. Antimicrobial activity of the extracted active compound was recorded as dose dependent bacteriostatic or bactericidal against antibiotic resistant clinical bacteria strains; methicillin-resistant Staphylococcus aureus (MRSA), vancomycin resistant Enterobacter faecium (VRE), and Acinetobacter baumanii. Minimum inhibitor concentration and minimum bactericidal concentrations were determined as lower than standard antibiotic streptomycin; 125 microg/ml and 250-1000 microg/ml, respectively. Active component was found as heat-stable.