A BROAD-SPECTRUM ARTIFICIAL SEAWATER MEDIUM FOR COASTAL AND OPEN OCEAN PHYTOPLANKTON
A BROAD-SPECTRUM ARTIFICIAL SEAWATER MEDIUM FOR COASTAL AND OPEN OCEAN PHYTOPLANKTON
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DOI：
10.1111/j.0022-3646.1980.00028.x
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发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
TAYLOR, FJR
中科院分区：
文献类型：
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作者：
HARRISON, PJ;WATERS, RE;TAYLOR, FJR
用来自11个不同藻类纲的83株沿海和开阔海洋浮游植物对一种新的人工海水培养基进行了试验。培养物通过4次转移进行，对于大多数种属，代表8周的时间。富集人工海水中仅3种无法维持，16种，主要来自Prymnesiophyceae和Dinophyceae，与在富集的天然海水中生长的那些相比，具有降低的最终细胞产量。由于77%的受试物种在富集的人工或天然海水中生长良好，95%以上的物种可以在人工培养基中维持，因此该配方适用于广泛的物种。用改进的富集溶液富集人工海水基;主要的改进是省略Tris和添加Si。通过在高压灭菌前降低pH值，对富集培养基进行高压灭菌而不沉淀。这是通过加入等摩尔量的Na-HCO 3和HCl来实现的，在加热过程中产生NaCl和CO2。当不使用pH缓冲液时，只能通过将人工海水基质高压灭菌为2种单独的盐溶液（Ca和Sr与CO 3 -2和SO 4 -2分离）、冷却、混合并无菌加入灭菌的富集溶液来避免沉淀。
A new artificial seawater medium was tested with 83 strains of coastal and open ocean phytoplankton from 11 different algal classes. The cultures were carried through 4 transfers, representing a period of 8 wk for most species. Only 3 species could not be maintained in the enriched artificial seawater, and 16 spp., mainly from the Prymnesiophyceae and Dinophyceae, had reduced final cell yields compared to those grown in enriched natural seawater. Since 77% of the species tested grew equally well in enriched artificial or natural seawater and more than 95% could be maintained in the artificial medium, this recipe is useful over a broad spectrum of species. The artificial seawater base was enriched with a modified enrichment solution; the primary modifications were the omission of Tris and the addition of Si. Enriched medium was autoclaved without precipitation by lowering the pH before autoclaving. This was accomplished by adding equimolar amounts of Na-HCO3 and HCl which produced NaCl and CO2 during the heating process. When no pH buffer was used, precipitation could only be avoided by autoclaving the artificial seawater base as 2 separate salt solutions (with Ca and Sr separated from CO3-2 and SO4-2), cooling, mixing and aseptically adding the sterilized enrichment solution.