Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host.
Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host.
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DOI：
10.1038/s41598-017-03804-8
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发表时间：
2017-06-16
影响因子：
4.6
通讯作者：
Wagner-Döbler I
中科院分区：
文献类型：
--
作者：
Deng ZL;Szafrański SP;Jarek M;Bhuju S;Wagner-Döbler I
牙周炎是一种在世界范围内极为普遍的疾病，是由复杂的益生菌群引起的。在这里，我们分析了来自所有生命领域以及健康和慢性牙周炎的人类宿主的牙周袋微生物群的转录活性。细菌在慢性牙周炎中富集了18个KEGG功能模块，包括细菌趋化性、鞭毛组装、III型分泌系统、III型CRISPR-Cas系统和双组分系统蛋白。这些功能的上调是由红色复合体病原体和候选病原体驱动的，如：嗜酸丝状因子、中间普雷沃氏菌、挑剔微杆菌和脓硒单胞菌。9个毒力因子显著上调，其中来自牙龈卟啉单胞菌和精氨酸支原体的精氨酸脱亚胺酶arcA上调。病毒和古细菌分别占估计mRNA总读数的0.1%和0.22%，牙周炎中原生动物牙龈内阿米巴（Entamoeba gingivalis）含量高。14个人类转录本在牙周炎中富集，包括一个铁结合蛋白基因，表明与微生物群争夺铁，以及与癌症相关的基因，即核仁磷酸化蛋白B23、钉蛋白重复结构域30b样蛋白和β烯醇酶。这些数据为体内基因表达水平提供了证据，证明了益生菌群对人类健康的潜在严重影响。
Periodontitis is an extremely prevalent disease worldwide and is driven by complex dysbiotic microbiota. Here we analyzed the transcriptional activity of the periodontal pocket microbiota from all domains of life as well as the human host in health and chronic periodontitis. Bacteria showed strong enrichment of 18 KEGG functional modules in chronic periodontitis, including bacterial chemotaxis, flagellar assembly, type III secretion system, type III CRISPR-Cas system, and two component system proteins. Upregulation of these functions was driven by the red-complex pathogens and candidate pathogens, e.g. Filifactor alocis, Prevotella intermedia, Fretibacterium fastidiosum and Selenomonas sputigena. Nine virulence factors were strongly up-regulated, among them the arginine deiminase arcA from Porphyromonas gingivalis and Mycoplasma arginini. Viruses and archaea accounted for about 0.1% and 0.22% of total putative mRNA reads, respectively, and a protozoan, Entamoeba gingivalis, was highly enriched in periodontitis. Fourteen human transcripts were enriched in periodontitis, including a gene for a ferric iron binding protein, indicating competition with the microbiota for iron, and genes associated with cancer, namely nucleolar phosphoprotein B23, ankyrin-repeat domain 30B-like protein and beta-enolase. The data provide evidence on the level of gene expression in vivo for the potentially severe impact of the dysbiotic microbiota on human health.