Resistance to fatigue of single muscle fibres from Xenopus related to succinate dehydrogenase and myofibrillar ATPase activities
Resistance to fatigue of single muscle fibres from Xenopus related to succinate dehydrogenase and myofibrillar ATPase activities
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非洲爪蟾单肌纤维的抗疲劳性与琥珀酸脱氢酶和肌原纤维 ATP 酶活性相关
DOI：
10.1113/expphysiol.1991.sp003526
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发表时间：
1991
影响因子：
2.7
通讯作者：
P. Diegenbach
中科院分区：
文献类型：
--
作者：
W. J. van der Laarse;J. Lannergren;P. Diegenbach
这篇报告描述了肌肉纤维的抗疲劳性如何与纤维最重要的ATP产生和ATP消耗反应相关。从非洲爪蟾蚓状肌中分离出12条完整的单个肌纤维。测定了它们对反复破伤风刺激引起的疲劳的抵抗力，以及它们的琥珀酸脱氢酶活性和钙刺激的肌纤维atp酶活性。采用定量组织化学方法测定酶活性。研究发现，对疲劳的抵抗力与琥珀酸脱氢酶活性（r = 0.83）和肌纤维atp酶活性（r =‐0.74）相关。抗疲劳能力与琥珀酸脱氢酶与肌纤维atp酶活性之比相关性最高（r = 0.93）。认为肌肉疲劳与细胞能量学密切相关。
This report describes how the resistance to fatigue of a muscle fibre relates to the fibre's most important ATP‐producing and ATP‐consuming reactions. Twelve intact single muscle fibres were dissected from lumbrical muscles of Xenopus laevis. Their resistance to fatigue induced by repeated tetanic stimulation was determined, as well as their succinate dehydrogenase activity and calcium‐stimulated myofibrillar ATPase activity. The enzyme activities were determined by means of quantitative histochemistry. It was found that resistance to fatigue correlates with succinate dehydrogenase activity (r = 0.83) and with myofibrillar ATPase activity (r = ‐0.74). The highest correlation was found between resistance to fatigue and the ratio of succinate dehydrogenase to myofibrillar ATPase activity (r = 0.93). It is concluded that muscular fatigue is closely related to cellular energetics.